Identification and Isolation of Regulatory B Cells in Mouse and Human

Madhvi Menon; Elizabeth C Rosser; Claudia Mauri

doi:10.1007/978-1-4939-8938-6_5

Identification and Isolation of Regulatory B Cells in Mouse and Human

Madhvi Menon, Elizabeth C Rosser, Claudia Mauri

Division of Immunology, Immunity to Infection and Respiratory Medicine

University College London (UCL)

Research output: Contribution to journal › Article › peer-review

Abstract

Regulatory B cells (Bregs) suppress immune response via the provision of IL-10. Due to the phenotypic heterogeneity of described Bregs, it is important to have standardized protocols for their isolation and identification. Previous work by our laboratory has shown that the immature B-cell populations in the murine spleen and human peripheral blood produce the highest levels of IL-10 on engagement of CD40, and can suppress pro-inflammatory T-cell differentiation. In this chapter, we describe the methods necessary for the isolation of this subset of Bregs and their activation via CD40 in vitro.

Original language	English
Pages (from-to)	55-66
Number of pages	12
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	1899
Early online date	17 Jan 2019
DOIs	https://doi.org/10.1007/978-1-4939-8938-6_5
Publication status	Published - 2019

Keywords

Animals
B-Lymphocytes, Regulatory/cytology
CD40 Antigens/immunology
Cell Separation/methods
Humans
Interleukin-10/immunology
Mice
T-Lymphocytes/cytology

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10.1007/978-1-4939-8938-6_5

https://discovery.ucl.ac.uk/id/eprint/10072553/

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title = "Identification and Isolation of Regulatory B Cells in Mouse and Human",

abstract = "Regulatory B cells (Bregs) suppress immune response via the provision of IL-10. Due to the phenotypic heterogeneity of described Bregs, it is important to have standardized protocols for their isolation and identification. Previous work by our laboratory has shown that the immature B-cell populations in the murine spleen and human peripheral blood produce the highest levels of IL-10 on engagement of CD40, and can suppress pro-inflammatory T-cell differentiation. In this chapter, we describe the methods necessary for the isolation of this subset of Bregs and their activation via CD40 in vitro.",

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year = "2019",

doi = "10.1007/978-1-4939-8938-6_5",

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T1 - Identification and Isolation of Regulatory B Cells in Mouse and Human

AU - Menon, Madhvi

AU - Rosser, Elizabeth C

AU - Mauri, Claudia

PY - 2019

Y1 - 2019

N2 - Regulatory B cells (Bregs) suppress immune response via the provision of IL-10. Due to the phenotypic heterogeneity of described Bregs, it is important to have standardized protocols for their isolation and identification. Previous work by our laboratory has shown that the immature B-cell populations in the murine spleen and human peripheral blood produce the highest levels of IL-10 on engagement of CD40, and can suppress pro-inflammatory T-cell differentiation. In this chapter, we describe the methods necessary for the isolation of this subset of Bregs and their activation via CD40 in vitro.

AB - Regulatory B cells (Bregs) suppress immune response via the provision of IL-10. Due to the phenotypic heterogeneity of described Bregs, it is important to have standardized protocols for their isolation and identification. Previous work by our laboratory has shown that the immature B-cell populations in the murine spleen and human peripheral blood produce the highest levels of IL-10 on engagement of CD40, and can suppress pro-inflammatory T-cell differentiation. In this chapter, we describe the methods necessary for the isolation of this subset of Bregs and their activation via CD40 in vitro.

KW - Animals

KW - B-Lymphocytes, Regulatory/cytology

KW - CD40 Antigens/immunology

KW - Cell Separation/methods

KW - Humans

KW - Interleukin-10/immunology

KW - Mice

KW - T-Lymphocytes/cytology

U2 - 10.1007/978-1-4939-8938-6_5

DO - 10.1007/978-1-4939-8938-6_5

M3 - Article

C2 - 30649765

SN - 1064-3745

VL - 1899

SP - 55

EP - 66

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

Identification and Isolation of Regulatory B Cells in Mouse and Human

Abstract

Keywords

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