Identification and Isolation of Regulatory B Cells in Mouse and Human

Madhvi Menon, Elizabeth C Rosser, Claudia Mauri

Research output: Contribution to journalArticlepeer-review


Regulatory B cells (Bregs) suppress immune response via the provision of IL-10. Due to the phenotypic heterogeneity of described Bregs, it is important to have standardized protocols for their isolation and identification. Previous work by our laboratory has shown that the immature B-cell populations in the murine spleen and human peripheral blood produce the highest levels of IL-10 on engagement of CD40, and can suppress pro-inflammatory T-cell differentiation. In this chapter, we describe the methods necessary for the isolation of this subset of Bregs and their activation via CD40 in vitro.

Original languageEnglish
Pages (from-to)55-66
Number of pages12
JournalMethods in molecular biology (Clifton, N.J.)
Early online date17 Jan 2019
Publication statusPublished - 2019


  • Animals
  • B-Lymphocytes, Regulatory/cytology
  • CD40 Antigens/immunology
  • Cell Separation/methods
  • Humans
  • Interleukin-10/immunology
  • Mice
  • T-Lymphocytes/cytology


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