Identification of a Biomarker Panel for Early Detection of Lung Cancer Patients

Bethany Geary; Michael J. Walker; Joseph T. Snow; Dave Lee; Maria Pernemalm; Saeedeh Maleki-Dizaji; Narges Azadbakht; Sophia Apostolidou; Julie Barnes; Piotr Krysiak; Rajesh Shah; Richard Booton; Caroline Dive; Philip A. Crosbie; Anthony D. Whetton

doi:10.1021/acs.jproteome.9b00287

Identification of a Biomarker Panel for Early Detection of Lung Cancer Patients

Bethany Geary, Michael J. Walker, Joseph T. Snow, Dave Lee, Maria Pernemalm, Saeedeh Maleki-Dizaji, Narges Azadbakht, Sophia Apostolidou, Julie Barnes, Piotr Krysiak, Rajesh Shah, Richard Booton, Caroline Dive, Philip A. Crosbie, Anthony D. Whetton

Research output: Contribution to journal › Article › peer-review

Abstract

Lung cancer is the most common cause of cancer-related mortality worldwide, characterized by late clinical presentation (49–53% of patients are diagnosed at stage IV) and consequently poor outcomes. One challenge in identifying biomarkers of early disease is the collection of samples from patients prior to symptomatic presentation. We used blood collected during surgical resection of lung tumors in an iTRAQ isobaric tagging experiment to identify proteins effluxing from tumors into pulmonary veins. Forty proteins were identified as having an increased abundance in the vein draining from the tumor compared to “healthy” pulmonary veins. These protein markers were then assessed in a second cohort that utilized the mass spectrometry (MS) technique: Sequential window acquisition of all theoretical fragment ion spectra (SWATH) MS. SWATH-MS was used to measure proteins in serum samples taken from 25 patients

Original language	English
Pages (from-to)	3369-3382
Number of pages	14
Journal	Journal of Proteome Research
Volume	18
Issue number	9
Early online date	13 Aug 2019
DOIs	https://doi.org/10.1021/acs.jproteome.9b00287
Publication status	Published - 2019

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Manchester Cancer Research Centre

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10.1021/acs.jproteome.9b00287

https://discovery.ucl.ac.uk/id/eprint/10081406/

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Geary, B., Walker, M. J., Snow, J. T., Lee, D., Pernemalm, M., Maleki-Dizaji, S., Azadbakht, N., Apostolidou, S., Barnes, J., Krysiak, P., Shah, R., Booton, R., Dive, C., Crosbie, P. A., & Whetton, A. D. (2019). Identification of a Biomarker Panel for Early Detection of Lung Cancer Patients. Journal of Proteome Research, 18(9), 3369-3382. https://doi.org/10.1021/acs.jproteome.9b00287

@article{43d5444e043a470b87389715843c7564,

title = "Identification of a Biomarker Panel for Early Detection of Lung Cancer Patients",

abstract = "Lung cancer is the most common cause of cancer-related mortality worldwide, characterized by late clinical presentation (49–53\% of patients are diagnosed at stage IV) and consequently poor outcomes. One challenge in identifying biomarkers of early disease is the collection of samples from patients prior to symptomatic presentation. We used blood collected during surgical resection of lung tumors in an iTRAQ isobaric tagging experiment to identify proteins effluxing from tumors into pulmonary veins. Forty proteins were identified as having an increased abundance in the vein draining from the tumor compared to “healthy” pulmonary veins. These protein markers were then assessed in a second cohort that utilized the mass spectrometry (MS) technique: Sequential window acquisition of all theoretical fragment ion spectra (SWATH) MS. SWATH-MS was used to measure proteins in serum samples taken from 25 patients ",

author = "Bethany Geary and Walker, \{Michael J.\} and Snow, \{Joseph T.\} and Dave Lee and Maria Pernemalm and Saeedeh Maleki-Dizaji and Narges Azadbakht and Sophia Apostolidou and Julie Barnes and Piotr Krysiak and Rajesh Shah and Richard Booton and Caroline Dive and Crosbie, \{Philip A.\} and Whetton, \{Anthony D.\}",

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year = "2019",

doi = "10.1021/acs.jproteome.9b00287",

language = "English",

volume = "18",

pages = "3369--3382",

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publisher = "American Chemical Society",

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Geary, B, Walker, MJ, Snow, JT, Lee, D, Pernemalm, M, Maleki-Dizaji, S, Azadbakht, N, Apostolidou, S, Barnes, J, Krysiak, P, Shah, R, Booton, R, Dive, C, Crosbie, PA & Whetton, AD 2019, 'Identification of a Biomarker Panel for Early Detection of Lung Cancer Patients', Journal of Proteome Research, vol. 18, no. 9, pp. 3369-3382. https://doi.org/10.1021/acs.jproteome.9b00287

TY - JOUR

T1 - Identification of a Biomarker Panel for Early Detection of Lung Cancer Patients

AU - Geary, Bethany

AU - Walker, Michael J.

AU - Snow, Joseph T.

AU - Lee, Dave

AU - Pernemalm, Maria

AU - Maleki-Dizaji, Saeedeh

AU - Azadbakht, Narges

AU - Apostolidou, Sophia

AU - Barnes, Julie

AU - Krysiak, Piotr

AU - Shah, Rajesh

AU - Booton, Richard

AU - Dive, Caroline

AU - Crosbie, Philip A.

AU - Whetton, Anthony D.

N1 - PMID: 31408348

PY - 2019

Y1 - 2019

N2 - Lung cancer is the most common cause of cancer-related mortality worldwide, characterized by late clinical presentation (49–53% of patients are diagnosed at stage IV) and consequently poor outcomes. One challenge in identifying biomarkers of early disease is the collection of samples from patients prior to symptomatic presentation. We used blood collected during surgical resection of lung tumors in an iTRAQ isobaric tagging experiment to identify proteins effluxing from tumors into pulmonary veins. Forty proteins were identified as having an increased abundance in the vein draining from the tumor compared to “healthy” pulmonary veins. These protein markers were then assessed in a second cohort that utilized the mass spectrometry (MS) technique: Sequential window acquisition of all theoretical fragment ion spectra (SWATH) MS. SWATH-MS was used to measure proteins in serum samples taken from 25 patients

AB - Lung cancer is the most common cause of cancer-related mortality worldwide, characterized by late clinical presentation (49–53% of patients are diagnosed at stage IV) and consequently poor outcomes. One challenge in identifying biomarkers of early disease is the collection of samples from patients prior to symptomatic presentation. We used blood collected during surgical resection of lung tumors in an iTRAQ isobaric tagging experiment to identify proteins effluxing from tumors into pulmonary veins. Forty proteins were identified as having an increased abundance in the vein draining from the tumor compared to “healthy” pulmonary veins. These protein markers were then assessed in a second cohort that utilized the mass spectrometry (MS) technique: Sequential window acquisition of all theoretical fragment ion spectra (SWATH) MS. SWATH-MS was used to measure proteins in serum samples taken from 25 patients

U2 - 10.1021/acs.jproteome.9b00287

DO - 10.1021/acs.jproteome.9b00287

M3 - Article

SN - 1535-3893

VL - 18

SP - 3369

EP - 3382

JO - Journal of Proteome Research

JF - Journal of Proteome Research

IS - 9

ER -

Identification of a Biomarker Panel for Early Detection of Lung Cancer Patients

Abstract

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UN SDGs

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