Identification of a good C-MYC antisense oligodeoxynucleotide target site and the inactivity at this site of novel NCH triplet - Targeting ribozymes

Richard V. Giles; David G. Spiller; Richard E. Clark; David M. Tidd

Identification of a good C-MYC antisense oligodeoxynucleotide target site and the inactivity at this site of novel NCH triplet - Targeting ribozymes

Richard V. Giles, David G. Spiller, Richard E. Clark, David M. Tidd

Research output: Contribution to journal › Article › peer-review

Abstract

A region of c-myc mRNA was identified which permitted very efficient antisense effects to be achieved in living cells using chimeric methylphosphonate phosphodiester antisense effectors. Novel inosine - containing ribozymes (which cleave after NCH triplets) were directed to an ACA triplet within this region and delivered into living cells. No ribozyme intracellular activity could be identified. Very low ribozyme function was also observed in in vitro assays using a 1700nt substrate RNA.

Original language	English
Pages (from-to)	1935-1944
Number of pages	9
Journal	Nucleosides and Nucleotides
Volume	18
Issue number	9
Publication status	Published - 1999

Cite this

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title = "Identification of a good C-MYC antisense oligodeoxynucleotide target site and the inactivity at this site of novel NCH triplet - Targeting ribozymes",

abstract = "A region of c-myc mRNA was identified which permitted very efficient antisense effects to be achieved in living cells using chimeric methylphosphonate phosphodiester antisense effectors. Novel inosine - containing ribozymes (which cleave after NCH triplets) were directed to an ACA triplet within this region and delivered into living cells. No ribozyme intracellular activity could be identified. Very low ribozyme function was also observed in in vitro assays using a 1700nt substrate RNA.",

author = "Giles, {Richard V.} and Spiller, {David G.} and Clark, {Richard E.} and Tidd, {David M.}",

year = "1999",

language = "English",

volume = "18",

pages = "1935--1944",

journal = "Nucleosides and Nucleotides",

issn = "0732-8311",

publisher = "Marcel Dekker Inc",

number = "9",

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TY - JOUR

T1 - Identification of a good C-MYC antisense oligodeoxynucleotide target site and the inactivity at this site of novel NCH triplet - Targeting ribozymes

AU - Giles, Richard V.

AU - Spiller, David G.

AU - Clark, Richard E.

AU - Tidd, David M.

PY - 1999

Y1 - 1999

N2 - A region of c-myc mRNA was identified which permitted very efficient antisense effects to be achieved in living cells using chimeric methylphosphonate phosphodiester antisense effectors. Novel inosine - containing ribozymes (which cleave after NCH triplets) were directed to an ACA triplet within this region and delivered into living cells. No ribozyme intracellular activity could be identified. Very low ribozyme function was also observed in in vitro assays using a 1700nt substrate RNA.

AB - A region of c-myc mRNA was identified which permitted very efficient antisense effects to be achieved in living cells using chimeric methylphosphonate phosphodiester antisense effectors. Novel inosine - containing ribozymes (which cleave after NCH triplets) were directed to an ACA triplet within this region and delivered into living cells. No ribozyme intracellular activity could be identified. Very low ribozyme function was also observed in in vitro assays using a 1700nt substrate RNA.

M3 - Article

SN - 0732-8311

VL - 18

SP - 1935

EP - 1944

JO - Nucleosides and Nucleotides

JF - Nucleosides and Nucleotides

IS - 9

ER -

Identification of a good C-MYC antisense oligodeoxynucleotide target site and the inactivity at this site of novel NCH triplet - Targeting ribozymes

Abstract

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