Identification of alternatively spliced variants of type II procollagen in vitreous

P. N. Bishop, A. J. Reardon, D. McLeod, S. Ayad

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    Adult and foetal bovine vitreous were analyzed for the presence of unprocessed and partially processed forms of type II procollagen. Type II procollagen can exist in two alternatively spliced forms: a short form which lacks a large part of the N-propeptide due to the splicing out of exon 2 and a long form in which exon 2 is expressed and which has a full-sized N-propeptide. Both slice variants were demonstrated and analyses of type II pN-collagen demonstrated that the long form predominated, the ratio of the long form to the short form being 5:1 in foetal vitreous and 1.5:1 in adult vitreous. In foetal vitreous 35% of the type II collagen extracted was in the pN form, but there was very little unprocessed type II procollagen or type II pC-collagen. In contrast, adult vitreous extracts contained relatively less type II pN-collagen, but contained larger amounts of type II pC-collagen and unprocessed type II procollagen.
    Original languageEnglish
    Pages (from-to)289-295
    Number of pages6
    JournalBiochemical and Biophysical Research Communications
    Issue number1
    Publication statusPublished - 1994


    • Alternative Splicing
    • Animals
    • Blotting, Western
    • Cattle
    • Electrophoresis, Polyacrylamide Gel
    • Embryonic and Fetal Development
    • Female
    • Fetus
    • Gestational Age
    • Pepsin A
    • Peptide Mapping
    • Pregnancy
    • biosynthesis: Procollagen
    • Research Support, Non-U.S. Gov't
    • Variation (Genetics)
    • embryology: Vitreous Body


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