Identification of genes involved in siderophore transport in Streptomyces coelicolor A3(2)

Robert Bunet, Anita Brock, Hans Ulrich Rexer, Eriko Takano

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The potential iron siderophore transporter genes have been determined from the genome sequence of Streptomyces coelicolor A3(2). One of these gene clusters, cdtABC, was disrupted and characterized to determine its role in the uptake of the siderophores produced by S. coelicolor. Resistance to the siderophore-like antibiotics, salmycin and albomycin, was tested in the parent and cdtABC mutant, showing that the parent, but not the mutant, was sensitive to salmycin, while both were resistant to albomycin. Ferrioxamine competition assays against salmycin suggest that the uptake of salmycin is via a ferrioxamine transport system. However, Fe-55 ferrioxamine B uptake experiments did not reveal any difference between the parent and mutant. This suggests that CdtABC specifically transports salmycin, while ferrioxamine uptake maybe substituted by another transport system. © 2006 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved.
    Original languageEnglish
    Pages (from-to)57-64
    Number of pages7
    JournalFEMS microbiology letters
    Volume262
    Issue number1
    DOIs
    Publication statusPublished - Sep 2006

    Keywords

    • ABC transporter
    • fep homologues
    • Siderophore
    • Streptomyces

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