Identification of lipocalin and apolipoprotein A1 as biomarkers of chronic obstructive pulmonary disease

Benjamin L. Nicholas, Paul Skipp, Sheila Barton, Dave Singh, Dinesh Bagmane, Richard Mould, Gilbert Angco, Jon Ward, Binita Guha-Niyogi, Susan Wilson, Peter Howarth, Donna E. Davies, Stephen Rennard, C. David O'Connor, Ratko Djukanović

    Research output: Contribution to journalArticlepeer-review


    Rationale: Much effort is being made to discover noninvasive biomarkers of chronic airway disease that might enable better management, predict prognosis, and provide new therapeutic targets. Objectives: To undertake a comprehensive, unbiased proteomic analysis of induced sputum and identify novel noninvasive biomarkers for chronic obstructive pulmonary disease (COPD). Methods: Induced sputum was obtained from patients with COPD with a spectrum of disease severity and from control subjects. Two dimensional gel electrophoresis and mass spectrometric identification of differentially expressed proteins were first applied to induced sputum from patients with GOLD stage 2 COPD and healthy smoker control subjects. Initial results thus obtained were validated by a combination of immunoassays (Western blotting and ELISA) applied to a large subject cohort. The biomarkers were localized to bronchial mucosa by immunohistochemistry. Measurements and Main Results: Of 1,325 individual protein spots identified, 37 were quantitatively and 3 qualitatively different between the two groups (P
    Original languageEnglish
    Pages (from-to)1049-1060
    Number of pages11
    JournalAmerican Journal of Respiratory and Critical Care Medicine
    Issue number10
    Publication statusPublished - 15 May 2010


    • Biomarkers
    • Chronic obstructive pulmonary disease
    • Induced sputum
    • Proteome
    • Two-dimensional polyacrylamide gel electrophoresis


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