Imaging element distribution and speciation in plant cells

F J Zhao; K L Moore; E Lombi; Y G Zhu

doi:10.1016/j.tplants.2013.12.001

Imaging element distribution and speciation in plant cells

F J Zhao, K L Moore, E Lombi, Y G Zhu

Research output: Contribution to journal › Article › peer-review

Abstract

To maintain cellular homeostasis, concentrations, chemical speciation, and localization of mineral nutrients and toxic trace elements need to be regulated. Imaging the cellular and subcellular localization of elements and measuring their in situ chemical speciation are challenging tasks that can be undertaken using synchrotron-based techniques, such as X-ray fluorescence and X-ray absorption spectrometry, and mass spectrometry-based techniques, such as secondary ion mass spectrometry and laser-ablation inductively coupled plasma mass spectrometry. We review the advantages and limitations of these techniques, and discuss examples of their applications, which have revealed highly heterogeneous distribution patterns of elements in different cell types, often varying in chemical speciation. Combining these techniques with molecular genetic approaches can unravel functions of genes involved in element homeostasis. © 2013 Elsevier Ltd.

Original language	English
Pages (from-to)	183-192
Number of pages	10
Journal	Trends in Plant Science
Volume	19
Issue number	3
DOIs	https://doi.org/10.1016/j.tplants.2013.12.001
Publication status	Published - 2014

Keywords

Chemical speciation
Plant cells
Synchrotron-based techniques
mass spectrometry
metabolism
plant cell
review
spectrometry
Spectrometry, X-Ray Emission

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10.1016/j.tplants.2013.12.001

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Cite this

@article{f467a8ccdbe7456da3f0fbf9fd2a3a6f,

title = "Imaging element distribution and speciation in plant cells",

abstract = "To maintain cellular homeostasis, concentrations, chemical speciation, and localization of mineral nutrients and toxic trace elements need to be regulated. Imaging the cellular and subcellular localization of elements and measuring their in situ chemical speciation are challenging tasks that can be undertaken using synchrotron-based techniques, such as X-ray fluorescence and X-ray absorption spectrometry, and mass spectrometry-based techniques, such as secondary ion mass spectrometry and laser-ablation inductively coupled plasma mass spectrometry. We review the advantages and limitations of these techniques, and discuss examples of their applications, which have revealed highly heterogeneous distribution patterns of elements in different cell types, often varying in chemical speciation. Combining these techniques with molecular genetic approaches can unravel functions of genes involved in element homeostasis. {\textcopyright} 2013 Elsevier Ltd.",

keywords = "Chemical speciation, Plant cells, Synchrotron-based techniques, mass spectrometry, metabolism, plant cell, review, spectrometry, Spectrometry, X-Ray Emission",

author = "Zhao, {F J} and Moore, {K L} and E Lombi and Zhu, {Y G}",

note = "Cited By :7 Export Date: 26 January 2015 CODEN: TPSCF Correspondence Address: Zhao, F.-J.; National Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Lab. of Plant Nutrition and Fertili. in Low-Middle Reaches of the Yangtze River, Min. of Agri., College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China; email: [email protected] References: Marschner, P., (2012) Marschner's Mineral Nutrition of Higher Plants, , Academic Press; Clemens, S., Plant science: the key to preventing slow cadmium poisoning (2013) Trends Plant Sci., 18, pp. 92-99; Zhao, F.J., Arsenic as a food-chain contaminant: mechanisms of plant uptake and metabolism and mitigation strategies (2010) Annu. Rev. Plant Biol., 61, pp. 535-559; White, P.J., Broadley, M.R., Biofortification of crops with seven mineral elements often lacking in human diets - iron, zinc, copper, calcium, magnesium, selenium and iodine (2009) New Phytol., 182, pp. 49-84; von Wir{\'e}n, N., Grand challenges in plant nutrition (2011) Front. Plant Sci., 2, p. 4; Salt, D.E., Ionomics and the study of the plant ionome (2008) Annu. Rev. Plant Biol., 59, pp. 709-733; Conn, S., Gilliham, M., Comparative physiology of elemental distributions in plants (2010) Ann. Bot., 105, pp. 1081-1102; K{\"u}pper, H., Cellular compartmentation of zinc in leaves of the hyperaccumulator Thlaspi caerulescens (1999) Plant Physiol., 119, pp. 305-311; Vogel-Mikus, K., Comparison of essential and non-essential element distribution in leaves of the Cd/Zn hyperaccumulator Thlaspi praecox as revealed by micro-PIXE (2008) Plant Cell Environ., 31, pp. 1484-1496; Sarret, G., Use of synchrotron-based techniques to elucidate metal uptake and metabolism in plants (2013) Adv. Agron., 119, pp. 1-82; Lombi, E., Susini, J., Synchrotron-based techniques for plant and soil science: opportunities, challenges and future perspectives (2009) Plant Soil, 320, pp. 1-35; Donner, E., Mapping element distributions in plant tissues using synchrotron X-ray fluorescence techniques (2013) Plant Mineral Nutrients: Methods and Protocols. Methods in Molecular Biology (Vol. 953), pp. 143-159. , Springer, F.J.M. Maathuis (Ed.); Punshon, T., The role of CAX1 and CAX3 in elemental distribution and abundance in Arabidopsis seed (2012) Plant Physiol., 158, pp. 352-362; Moore, K.L., Combined NanoSIMS and synchrotron X-ray fluorescence reveals distinct cellular and subcellular distribution patterns of trace elements in rice tissues (2014) New Phytol., 201, pp. 104-115; de Jonge, M.D., Vogt, S., Hard X-ray fluorescence tomography - an emerging tool for structural visualization (2010) Curr. Opin. Struct. Biol., 20, pp. 606-614; Lombi, E., In situ analysis of metal(loid)s in plants: state of the art and artefacts (2011) Environ. Exp. Bot., 72, pp. 3-17; Wang, P., In situ speciation and distribution of toxic selenium in hydrated roots of cowpea (2013) Plant Physiol., 163, pp. 407-418; George, G.N., X-ray-induced photo-chemistry and X-ray absorption spectroscopy of biological samples (2012) J. Synchr. Radiat., 19, pp. 875-886; Lombi, E., Trends in hard X-ray fluorescence mapping: environmental applications in the age of fast detectors (2011) Anal. Bioanal. Chem., 400, pp. 1637-1644; Metzner, R., Imaging nutrient distributions in plant tissue using time-of-flight secondary ion mass spectrometry and scanning electron microscopy (2008) Plant Physiol., 147, pp. 1774-1787; Moore, K.L., Elemental imaging at the nanoscale: NanoSIMS and complementary techniques for element localisation in plants (2012) Anal. Bioanal. Chem., 402, pp. 3263-3273; Smart, K.E., High-resolution elemental localization in vacuolate plant cells by nanoscale secondary ion mass spectrometry (2010) Plant J., 63, pp. 870-879; Chandra, S., Subcellular imaging by dynamic SIMS ion microscopy (2000) Anal. Chem., 72, pp. 104a-114a; Moore, K.L., NanoSIMS analysis reveals contrasting patterns of arsenic and silicon localization in rice roots (2011) Plant Physiol., 156, pp. 913-924; Wu, B., Becker, J.S., Imaging techniques for elements and element species in plant science (2012) Metallomics, 4, pp. 403-416; Becker, J.S., Scaling down the bioimaging of metals by laser microdissection inductively coupled plasma mass spectrometry (LMD-ICP-MS) (2010) Int. J. Mass Spectrom., 294, pp. 1-6; Fukuda, N., Micro X-ray fluorescence imaging and micro X-ray absorption spectroscopy of cadmium hyper-accumulating plant, Arabidopsis halleri ssp gemmifera, using high-energy synchrotron radiation (2008) J. Anal. Atom. Spectrom., 23, pp. 1068-1075; Sarret, G., Forms of zinc accumulated in the hyperaccumulator Arabidopsis halleri (2002) Plant Physiol., 130, pp. 1815-1826; McNear, D.H., Application of quantitative fluorescence and absorption-edge computed microtomography to image metal compartmentalization in Alyssum murale (2005) Environ. Sci. Technol., 39, pp. 2210-2218; Freeman, J.L., Spatial imaging, speciation, and quantification of selenium in the hyperaccumulator plants Astragalus bisulcatus and Stanleya pinnata (2006) Plant Physiol., 142, pp. 124-134; Pickering, I.J., Localizing the biochemical transformations of arsenate in a hyperaccumulating fern (2006) Environ. Sci. Technol., 40, pp. 5010-5014; Scheckel, K.G., Synchrotron X-ray absorption-edge computed microtomography imaging of thallium compartmentalization in Iberis intermedia (2007) Plant Soil, 290, pp. 51-60; Blute, N.K., Arsenic sequestration by ferric iron plaque on cattail roots (2004) Environ. Sci. Technol., 38, pp. 6074-6077; Seyfferth, A.L., Arsenic localization, speciation, and co-occurrence with iron on rice (Oryza sativa L.) roots having variable Fe coatings (2010) Environ. Sci. Technol., 44, pp. 8108-8113; Chen, Z., Direct evidence showing the effect of root surface iron plaque on arsenite and arsenate uptake into rice (Oryza sativa) roots (2005) New Phytol., 165, pp. 91-97; Kopittke, P.M., Examination of the distribution of arsenic in hydrated and fresh cowpea roots using two- and three-dimensional techniques (2012) Plant Physiol., 159, pp. 1149-1158; Lombi, E., Fast X-ray fluorescence microtomography of hydrated biological samples (2011) PLoS ONE, 6, pp. e20626; Kopittke, P.M., In situ distribution and speciation of toxic copper, nickel, and zinc in hydrated roots of cowpea (2011) Plant Physiol., 156, pp. 663-673; Kopittke, P.M., Distribution and speciation of Mn in hydrated roots of cowpea at levels inhibiting root growth (2013) Physiol. Plant., 147, pp. 453-464; Carey, A.M., Grain unloading of arsenic species in rice (2010) Plant Physiol., 152, pp. 309-319; Zhao, F.J., Arsenic translocation in rice investigated using radioactive 73As tracer (2012) Plant Soil, 350, pp. 413-420; Carey, A.M., Phloem transport of arsenic species from flag leaf to grain during grain filling (2011) New Phytol., 192, pp. 87-98; Lombi, E., Speciation and distribution of arsenic and localization of nutrients in rice grains (2009) New Phytol., 184, pp. 193-201; Zheng, M.Z., Differential toxicity and accumulation of inorganic and methylated arsenic in rice (2013) Plant Soil, 365, pp. 227-238; Moore, K.L., NanoSIMS analysis of arsenic and selenium in cereal grain (2010) New Phytol., 185, pp. 434-445; Van Belleghem, F., Subcellular localization of cadmium in roots and leaves of Arabidopsis thaliana (2007) New Phytol., 173, pp. 495-508; Isaure, M.P., Localization and chemical forms of cadmium in plant samples by combining analytical electron microscopy and X-ray spectromicroscopy (2006) Spectrochim. Acta B: Atom. Spectrom., 61, pp. 1242-1252; Yamaguchi, N., Role of the node in controlling traffic of cadmium, zinc, and manganese in rice (2012) J. Exp. Bot., 63, pp. 2729-2737; Zheng, L.Q., Physiological and transcriptome analysis of iron and phosphorus interaction in rice seedlings (2009) Plant Physiol., 151, pp. 262-274; Kim, S.A., Localization of iron in Arabidopsis seed requires the vacuolar membrane transporter VIT1 (2006) Science, 314, pp. 1295-1298; Heard, P.J., Determination of the elemental composition of mature wheat grain using a modified secondary ion mass spectrometer (SIMS) (2002) Plant J., 30, pp. 237-245; Moore, K.L., Localisation of iron in wheat grain using high resolution secondary ion mass spectrometry (2012) J. Cereal Sci., 55, pp. 183-187; Wang, Y.X., Stable isotope labelling and zinc distribution in grains studied by laser ablation ICP-MS in an ear culture system reveals zinc transport barriers during grain filling in wheat (2011) New Phytol., 189, pp. 428-437; Wirth, J., Rice endosperm iron biofortification by targeted and synergistic action of nicotianamine synthase and ferritin (2009) Plant Biotechnol. J., 7, pp. 631-644; Clode, P.L., In situ mapping of nutrient uptake in the rhizosphere using nanoscale secondary ion mass spectrometry (2009) Plant Physiol., 151, pp. 1751-1757; Jones, D.L., Competition between plant and bacterial cells at the microscale regulates the dynamics of nitrogen acquisition in wheat (Triticum aestivum) (2013) New Phytol., 200, pp. 796-807; Metzner, R., Tracing cationic nutrients from xylem into stem tissue of French bean by stable isotope tracers and cryo-secondary ion mass spectrometry (2010) Plant Physiol., 152, pp. 1030-1043; Metzner, R., Contrasting dynamics of water and mineral nutrients in stems shown by stable isotope tracers and cryo-SIMS (2010) Plant Cell Environ., 33, pp. 1393-1407; Mishra, S., Speciation and distribution of arsenic in the nonhyperaccumulator macrophyte Ceratophyllum demersum (2013) Plant Physiol., 163, pp. 1396-1408; Zhao, F.J., Arsenic uptake and metabolism in plants (2009) New Phytol., 181, pp. 777-794; Pickering, I.J., Reduction and coordination of arsenic in Indian mustard (2000) Plant Physiol., 122, pp. 1171-1177; Bluemlein, K., Can we trust mass spectrometry for determination of arsenic peptides in plants: comparison of LC-ICP-MS and LC-ES-MS/ICP-MS with XANES/EXAFS in analysis of Thunbergia alata (2008) Anal. Bioanal. Chem., 390, pp. 1739-1751; Castillo-Michel, H., Localization and speciation of arsenic in soil and desert plant Parkinsonia florida using mu XRF and mu XANES (2011) Environ. Sci. Technol., 45, pp. 7848-7854; Webb, S.M., XAS speciation of arsenic in a hyper-accumulating fern (2003) Environ. Sci. Technol., 37, pp. 754-760; Lombi, E., Arsenic distribution and speciation in the fronds of the hyperaccumulator Pteris vittata (2002) New Phytol., 156, pp. 195-203; Kopittke, P.M., Laterally resolved speciation of arsenic in roots of wheat and rice using fluorescence-XANES imaging (2013) New Phytol.; Maher, W., Measurement of inorganic arsenic species in rice after nitric acid extraction by HPLC-ICPMS: verification using XANES (2013) Environ. Sci. Technol., 47, pp. 5821-5827; Salt, D.E., Mechanisms of cadmium mobility and accumulation in Indian mustard (1995) Plant Physiol., 109, pp. 1427-1433; K{\"u}pper, H., Tissue- and age-dependent differences in the complexation of cadmium and zinc in the cadmium/zinc hyperaccumulator Thlaspi caerulescens (Ganges ecotype) revealed by X-ray absorption spectroscopy (2004) Plant Physiol., 134, pp. 748-757; Huguet, S., Cd speciation and localization in the hyperaccumulator Arabidopsis halleri (2012) Environ. Exp. Bot., 82, pp. 54-65; Tian, S.K., Cellular sequestration of cadmium in the hyperaccumulator plant species Sedum alfredii (2011) Plant Physiol., 157, pp. 1914-1925; Vogel-Mikus, K., Complexation of cadmium in seeds and vegetative tissues of the cadmium hyperaccumulator Thlaspi praecox as studied by X-ray absorption spectroscopy (2010) Plant Soil, 331, pp. 439-451; Salt, D.E., Zinc ligands in the metal hyperaccumulator Thlaspi caerulescens as determined using X-ray absorption spectroscopy (1999) Environ. Sci. Technol., 33, pp. 713-717; Sarret, G., Zinc distribution and speciation in Arabidopsis halleri × Arabidopsis lyrata progenies presenting various zinc accumulation capacities (2009) New Phytol., 184, pp. 581-595; Neal, A.L., Iron and zinc complexation in wild-type and ferritin-expressing wheat grain: implications for mineral transport into developing grain (2013) J. Biol. Inorg. Chem., 18, pp. 557-570; Ryan, B.M., Copper speciation and isotopic fractionation in plants: uptake and translocation mechanisms (2013) New Phytol., 199, pp. 367-378; Mijovilovich, A., Complexation and toxicity of copper in higher plants. II. Different mechanisms for copper versus cadmium detoxification in the copper-sensitive cadmium/zinc hyperaccumulator Thlaspi caerulescens (Ganges ecotype) (2009) Plant Physiol., 151, pp. 715-731; Pushie, M.J., The fictile coordination chemistry of cuprous-thiolate sites in copper chaperones (2012) Biochim. Biophys. Acta Bioenerg., 1817, pp. 938-947; K{\"u}pper, H., Complexation and toxicity of copper in higher plants. I. Characterization of copper accumulation, speciation, and toxicity in Crassula helmsii as a new copper accumulator (2009) Plant Physiol., 151, pp. 702-714; Young, L.W., A high-throughput determination of metal concentrations in whole intact Arabidopsis thaliana seeds using synchrotron-based X-ray fluorescence spectroscopy (2006) J. Synchr. Radiat., 13, pp. 304-313; Pickering, I.J., Quantitative, chemically specific imaging of selenium transformation in plants (2000) Proc. Natl. Acad. Sci. U.S.A., 97, pp. 10717-10722; Etschmann, B.E., Reduced As components in highly oxidized environments: evidence from full spectral XANES imaging using the Maia massively parallel detector (2010) Am. Mineral., 95, pp. 884-887; Wu, B., Becker, J.S., Imaging of elements and molecules in biological tissues and cells in the low-micrometer and nanometer range (2011) Int. J. Mass Spectrom., 307, pp. 112-122; Johnson, A.A.T., Constitutive overexpression of the OsNAS gene family reveals single-gene strategies for effective iron- and zinc-biofortification of rice endosperm (2011) PLoS ONE, 6, pp. e24476",

year = "2014",

doi = "10.1016/j.tplants.2013.12.001",

language = "English",

volume = "19",

pages = "183--192",

journal = "Trends in Plant Science",

issn = "1360-1385",

publisher = "Elsevier BV",

number = "3",

}

TY - JOUR

T1 - Imaging element distribution and speciation in plant cells

AU - Zhao, F J

AU - Moore, K L

AU - Lombi, E

AU - Zhu, Y G

N1 - Cited By :7 Export Date: 26 January 2015 CODEN: TPSCF Correspondence Address: Zhao, F.-J.; National Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Lab. of Plant Nutrition and Fertili. in Low-Middle Reaches of the Yangtze River, Min. of Agri., College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China; email: [email protected] References: Marschner, P., (2012) Marschner's Mineral Nutrition of Higher Plants, , Academic Press; Clemens, S., Plant science: the key to preventing slow cadmium poisoning (2013) Trends Plant Sci., 18, pp. 92-99; Zhao, F.J., Arsenic as a food-chain contaminant: mechanisms of plant uptake and metabolism and mitigation strategies (2010) Annu. Rev. Plant Biol., 61, pp. 535-559; White, P.J., Broadley, M.R., Biofortification of crops with seven mineral elements often lacking in human diets - iron, zinc, copper, calcium, magnesium, selenium and iodine (2009) New Phytol., 182, pp. 49-84; von Wirén, N., Grand challenges in plant nutrition (2011) Front. Plant Sci., 2, p. 4; Salt, D.E., Ionomics and the study of the plant ionome (2008) Annu. Rev. Plant Biol., 59, pp. 709-733; Conn, S., Gilliham, M., Comparative physiology of elemental distributions in plants (2010) Ann. Bot., 105, pp. 1081-1102; Küpper, H., Cellular compartmentation of zinc in leaves of the hyperaccumulator Thlaspi caerulescens (1999) Plant Physiol., 119, pp. 305-311; Vogel-Mikus, K., Comparison of essential and non-essential element distribution in leaves of the Cd/Zn hyperaccumulator Thlaspi praecox as revealed by micro-PIXE (2008) Plant Cell Environ., 31, pp. 1484-1496; Sarret, G., Use of synchrotron-based techniques to elucidate metal uptake and metabolism in plants (2013) Adv. Agron., 119, pp. 1-82; Lombi, E., Susini, J., Synchrotron-based techniques for plant and soil science: opportunities, challenges and future perspectives (2009) Plant Soil, 320, pp. 1-35; Donner, E., Mapping element distributions in plant tissues using synchrotron X-ray fluorescence techniques (2013) Plant Mineral Nutrients: Methods and Protocols. Methods in Molecular Biology (Vol. 953), pp. 143-159. , Springer, F.J.M. Maathuis (Ed.); Punshon, T., The role of CAX1 and CAX3 in elemental distribution and abundance in Arabidopsis seed (2012) Plant Physiol., 158, pp. 352-362; Moore, K.L., Combined NanoSIMS and synchrotron X-ray fluorescence reveals distinct cellular and subcellular distribution patterns of trace elements in rice tissues (2014) New Phytol., 201, pp. 104-115; de Jonge, M.D., Vogt, S., Hard X-ray fluorescence tomography - an emerging tool for structural visualization (2010) Curr. Opin. Struct. Biol., 20, pp. 606-614; Lombi, E., In situ analysis of metal(loid)s in plants: state of the art and artefacts (2011) Environ. Exp. Bot., 72, pp. 3-17; Wang, P., In situ speciation and distribution of toxic selenium in hydrated roots of cowpea (2013) Plant Physiol., 163, pp. 407-418; George, G.N., X-ray-induced photo-chemistry and X-ray absorption spectroscopy of biological samples (2012) J. Synchr. Radiat., 19, pp. 875-886; Lombi, E., Trends in hard X-ray fluorescence mapping: environmental applications in the age of fast detectors (2011) Anal. Bioanal. Chem., 400, pp. 1637-1644; Metzner, R., Imaging nutrient distributions in plant tissue using time-of-flight secondary ion mass spectrometry and scanning electron microscopy (2008) Plant Physiol., 147, pp. 1774-1787; Moore, K.L., Elemental imaging at the nanoscale: NanoSIMS and complementary techniques for element localisation in plants (2012) Anal. Bioanal. Chem., 402, pp. 3263-3273; Smart, K.E., High-resolution elemental localization in vacuolate plant cells by nanoscale secondary ion mass spectrometry (2010) Plant J., 63, pp. 870-879; Chandra, S., Subcellular imaging by dynamic SIMS ion microscopy (2000) Anal. Chem., 72, pp. 104a-114a; Moore, K.L., NanoSIMS analysis reveals contrasting patterns of arsenic and silicon localization in rice roots (2011) Plant Physiol., 156, pp. 913-924; Wu, B., Becker, J.S., Imaging techniques for elements and element species in plant science (2012) Metallomics, 4, pp. 403-416; Becker, J.S., Scaling down the bioimaging of metals by laser microdissection inductively coupled plasma mass spectrometry (LMD-ICP-MS) (2010) Int. J. Mass Spectrom., 294, pp. 1-6; Fukuda, N., Micro X-ray fluorescence imaging and micro X-ray absorption spectroscopy of cadmium hyper-accumulating plant, Arabidopsis halleri ssp gemmifera, using high-energy synchrotron radiation (2008) J. Anal. Atom. Spectrom., 23, pp. 1068-1075; Sarret, G., Forms of zinc accumulated in the hyperaccumulator Arabidopsis halleri (2002) Plant Physiol., 130, pp. 1815-1826; McNear, D.H., Application of quantitative fluorescence and absorption-edge computed microtomography to image metal compartmentalization in Alyssum murale (2005) Environ. Sci. Technol., 39, pp. 2210-2218; Freeman, J.L., Spatial imaging, speciation, and quantification of selenium in the hyperaccumulator plants Astragalus bisulcatus and Stanleya pinnata (2006) Plant Physiol., 142, pp. 124-134; Pickering, I.J., Localizing the biochemical transformations of arsenate in a hyperaccumulating fern (2006) Environ. Sci. Technol., 40, pp. 5010-5014; Scheckel, K.G., Synchrotron X-ray absorption-edge computed microtomography imaging of thallium compartmentalization in Iberis intermedia (2007) Plant Soil, 290, pp. 51-60; Blute, N.K., Arsenic sequestration by ferric iron plaque on cattail roots (2004) Environ. Sci. Technol., 38, pp. 6074-6077; Seyfferth, A.L., Arsenic localization, speciation, and co-occurrence with iron on rice (Oryza sativa L.) roots having variable Fe coatings (2010) Environ. Sci. Technol., 44, pp. 8108-8113; Chen, Z., Direct evidence showing the effect of root surface iron plaque on arsenite and arsenate uptake into rice (Oryza sativa) roots (2005) New Phytol., 165, pp. 91-97; Kopittke, P.M., Examination of the distribution of arsenic in hydrated and fresh cowpea roots using two- and three-dimensional techniques (2012) Plant Physiol., 159, pp. 1149-1158; Lombi, E., Fast X-ray fluorescence microtomography of hydrated biological samples (2011) PLoS ONE, 6, pp. e20626; Kopittke, P.M., In situ distribution and speciation of toxic copper, nickel, and zinc in hydrated roots of cowpea (2011) Plant Physiol., 156, pp. 663-673; Kopittke, P.M., Distribution and speciation of Mn in hydrated roots of cowpea at levels inhibiting root growth (2013) Physiol. Plant., 147, pp. 453-464; Carey, A.M., Grain unloading of arsenic species in rice (2010) Plant Physiol., 152, pp. 309-319; Zhao, F.J., Arsenic translocation in rice investigated using radioactive 73As tracer (2012) Plant Soil, 350, pp. 413-420; Carey, A.M., Phloem transport of arsenic species from flag leaf to grain during grain filling (2011) New Phytol., 192, pp. 87-98; Lombi, E., Speciation and distribution of arsenic and localization of nutrients in rice grains (2009) New Phytol., 184, pp. 193-201; Zheng, M.Z., Differential toxicity and accumulation of inorganic and methylated arsenic in rice (2013) Plant Soil, 365, pp. 227-238; Moore, K.L., NanoSIMS analysis of arsenic and selenium in cereal grain (2010) New Phytol., 185, pp. 434-445; Van Belleghem, F., Subcellular localization of cadmium in roots and leaves of Arabidopsis thaliana (2007) New Phytol., 173, pp. 495-508; Isaure, M.P., Localization and chemical forms of cadmium in plant samples by combining analytical electron microscopy and X-ray spectromicroscopy (2006) Spectrochim. Acta B: Atom. Spectrom., 61, pp. 1242-1252; Yamaguchi, N., Role of the node in controlling traffic of cadmium, zinc, and manganese in rice (2012) J. Exp. Bot., 63, pp. 2729-2737; Zheng, L.Q., Physiological and transcriptome analysis of iron and phosphorus interaction in rice seedlings (2009) Plant Physiol., 151, pp. 262-274; Kim, S.A., Localization of iron in Arabidopsis seed requires the vacuolar membrane transporter VIT1 (2006) Science, 314, pp. 1295-1298; Heard, P.J., Determination of the elemental composition of mature wheat grain using a modified secondary ion mass spectrometer (SIMS) (2002) Plant J., 30, pp. 237-245; Moore, K.L., Localisation of iron in wheat grain using high resolution secondary ion mass spectrometry (2012) J. Cereal Sci., 55, pp. 183-187; Wang, Y.X., Stable isotope labelling and zinc distribution in grains studied by laser ablation ICP-MS in an ear culture system reveals zinc transport barriers during grain filling in wheat (2011) New Phytol., 189, pp. 428-437; Wirth, J., Rice endosperm iron biofortification by targeted and synergistic action of nicotianamine synthase and ferritin (2009) Plant Biotechnol. J., 7, pp. 631-644; Clode, P.L., In situ mapping of nutrient uptake in the rhizosphere using nanoscale secondary ion mass spectrometry (2009) Plant Physiol., 151, pp. 1751-1757; Jones, D.L., Competition between plant and bacterial cells at the microscale regulates the dynamics of nitrogen acquisition in wheat (Triticum aestivum) (2013) New Phytol., 200, pp. 796-807; Metzner, R., Tracing cationic nutrients from xylem into stem tissue of French bean by stable isotope tracers and cryo-secondary ion mass spectrometry (2010) Plant Physiol., 152, pp. 1030-1043; Metzner, R., Contrasting dynamics of water and mineral nutrients in stems shown by stable isotope tracers and cryo-SIMS (2010) Plant Cell Environ., 33, pp. 1393-1407; Mishra, S., Speciation and distribution of arsenic in the nonhyperaccumulator macrophyte Ceratophyllum demersum (2013) Plant Physiol., 163, pp. 1396-1408; Zhao, F.J., Arsenic uptake and metabolism in plants (2009) New Phytol., 181, pp. 777-794; Pickering, I.J., Reduction and coordination of arsenic in Indian mustard (2000) Plant Physiol., 122, pp. 1171-1177; Bluemlein, K., Can we trust mass spectrometry for determination of arsenic peptides in plants: comparison of LC-ICP-MS and LC-ES-MS/ICP-MS with XANES/EXAFS in analysis of Thunbergia alata (2008) Anal. Bioanal. Chem., 390, pp. 1739-1751; Castillo-Michel, H., Localization and speciation of arsenic in soil and desert plant Parkinsonia florida using mu XRF and mu XANES (2011) Environ. Sci. Technol., 45, pp. 7848-7854; Webb, S.M., XAS speciation of arsenic in a hyper-accumulating fern (2003) Environ. Sci. Technol., 37, pp. 754-760; Lombi, E., Arsenic distribution and speciation in the fronds of the hyperaccumulator Pteris vittata (2002) New Phytol., 156, pp. 195-203; Kopittke, P.M., Laterally resolved speciation of arsenic in roots of wheat and rice using fluorescence-XANES imaging (2013) New Phytol.; Maher, W., Measurement of inorganic arsenic species in rice after nitric acid extraction by HPLC-ICPMS: verification using XANES (2013) Environ. Sci. Technol., 47, pp. 5821-5827; Salt, D.E., Mechanisms of cadmium mobility and accumulation in Indian mustard (1995) Plant Physiol., 109, pp. 1427-1433; Küpper, H., Tissue- and age-dependent differences in the complexation of cadmium and zinc in the cadmium/zinc hyperaccumulator Thlaspi caerulescens (Ganges ecotype) revealed by X-ray absorption spectroscopy (2004) Plant Physiol., 134, pp. 748-757; Huguet, S., Cd speciation and localization in the hyperaccumulator Arabidopsis halleri (2012) Environ. Exp. Bot., 82, pp. 54-65; Tian, S.K., Cellular sequestration of cadmium in the hyperaccumulator plant species Sedum alfredii (2011) Plant Physiol., 157, pp. 1914-1925; Vogel-Mikus, K., Complexation of cadmium in seeds and vegetative tissues of the cadmium hyperaccumulator Thlaspi praecox as studied by X-ray absorption spectroscopy (2010) Plant Soil, 331, pp. 439-451; Salt, D.E., Zinc ligands in the metal hyperaccumulator Thlaspi caerulescens as determined using X-ray absorption spectroscopy (1999) Environ. Sci. Technol., 33, pp. 713-717; Sarret, G., Zinc distribution and speciation in Arabidopsis halleri × Arabidopsis lyrata progenies presenting various zinc accumulation capacities (2009) New Phytol., 184, pp. 581-595; Neal, A.L., Iron and zinc complexation in wild-type and ferritin-expressing wheat grain: implications for mineral transport into developing grain (2013) J. Biol. Inorg. 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PY - 2014

Y1 - 2014

N2 - To maintain cellular homeostasis, concentrations, chemical speciation, and localization of mineral nutrients and toxic trace elements need to be regulated. Imaging the cellular and subcellular localization of elements and measuring their in situ chemical speciation are challenging tasks that can be undertaken using synchrotron-based techniques, such as X-ray fluorescence and X-ray absorption spectrometry, and mass spectrometry-based techniques, such as secondary ion mass spectrometry and laser-ablation inductively coupled plasma mass spectrometry. We review the advantages and limitations of these techniques, and discuss examples of their applications, which have revealed highly heterogeneous distribution patterns of elements in different cell types, often varying in chemical speciation. Combining these techniques with molecular genetic approaches can unravel functions of genes involved in element homeostasis. © 2013 Elsevier Ltd.

AB - To maintain cellular homeostasis, concentrations, chemical speciation, and localization of mineral nutrients and toxic trace elements need to be regulated. Imaging the cellular and subcellular localization of elements and measuring their in situ chemical speciation are challenging tasks that can be undertaken using synchrotron-based techniques, such as X-ray fluorescence and X-ray absorption spectrometry, and mass spectrometry-based techniques, such as secondary ion mass spectrometry and laser-ablation inductively coupled plasma mass spectrometry. We review the advantages and limitations of these techniques, and discuss examples of their applications, which have revealed highly heterogeneous distribution patterns of elements in different cell types, often varying in chemical speciation. Combining these techniques with molecular genetic approaches can unravel functions of genes involved in element homeostasis. © 2013 Elsevier Ltd.

KW - Chemical speciation

KW - Plant cells

KW - Synchrotron-based techniques

KW - mass spectrometry

KW - metabolism

KW - plant cell

KW - review

KW - spectrometry

KW - Spectrometry, X-Ray Emission

U2 - 10.1016/j.tplants.2013.12.001

DO - 10.1016/j.tplants.2013.12.001

M3 - Article

SN - 1360-1385

VL - 19

SP - 183

EP - 192

JO - Trends in Plant Science

JF - Trends in Plant Science

IS - 3

ER -

Imaging element distribution and speciation in plant cells

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