Imaging ToF-SIMS and synchrotron-based FT-IR microspectroscopic studies of prostate cancer cell lines

E. Gazi; N. P. Lockyer; J. C. Vickerman; P. Gardner; J. Dwyer; C. A. Hart; M. D. Brown; N. W. Clarke; J. Miyan

doi:10.1016/j.apsusc.2004.03.170

Imaging ToF-SIMS and synchrotron-based FT-IR microspectroscopic studies of prostate cancer cell lines

E. Gazi, N. P. Lockyer, J. C. Vickerman, P. Gardner, J. Dwyer, C. A. Hart, M. D. Brown, N. W. Clarke, J. Miyan

Division of Cancer Sciences (L5)

Research output: Contribution to journal › Article › peer-review

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Abstract

Imaging ToF-SIMS and synchrotron-based Fourier transform infrared (SR-FT-IR) microspectroscopy have been used to obtain chemical information from individual cells derived from human prostate cancer (CaP). ToF-SIMS imaging of molecular signals characteristic of membrane bound phospholipids are used to elucidate different fracture planes within individual freeze-fractured CaP cells. The localisation of Cu within the cytoplasm of cancer cells is consistent with increased metastatic potential. Line scans across CaP cells using SR-FT-IR microspectroscopy provide complimentary information on the localisation (±1μm) of lipid and protein domains. This combined analytical approach offers a novel means of characterising individual CaP cells and investigating the biochemical basis of disease progression and metastases. © 2004 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	452-456
Number of pages	4
Journal	Applied Surface Science
Volume	231-232
DOIs	https://doi.org/10.1016/j.apsusc.2004.03.170
Publication status	Published - 15 Jun 2004

Keywords

Cell lines
FT-IR microspectroscopy
Imaging
Prostate cancer
ToF-SIMS

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.apsusc.2004.03.170

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@article{4843fa0074014547a725ace134eae7ea,

title = "Imaging ToF-SIMS and synchrotron-based FT-IR microspectroscopic studies of prostate cancer cell lines",

abstract = "Imaging ToF-SIMS and synchrotron-based Fourier transform infrared (SR-FT-IR) microspectroscopy have been used to obtain chemical information from individual cells derived from human prostate cancer (CaP). ToF-SIMS imaging of molecular signals characteristic of membrane bound phospholipids are used to elucidate different fracture planes within individual freeze-fractured CaP cells. The localisation of Cu within the cytoplasm of cancer cells is consistent with increased metastatic potential. Line scans across CaP cells using SR-FT-IR microspectroscopy provide complimentary information on the localisation (±1μm) of lipid and protein domains. This combined analytical approach offers a novel means of characterising individual CaP cells and investigating the biochemical basis of disease progression and metastases. {\textcopyright} 2004 Elsevier B.V. All rights reserved.",

keywords = "Cell lines, FT-IR microspectroscopy, Imaging, Prostate cancer, ToF-SIMS",

author = "E. Gazi and Lockyer, {N. P.} and Vickerman, {J. C.} and P. Gardner and J. Dwyer and Hart, {C. A.} and Brown, {M. D.} and Clarke, {N. W.} and J. Miyan",

year = "2004",

month = jun,

day = "15",

doi = "10.1016/j.apsusc.2004.03.170",

language = "English",

volume = "231-232",

pages = "452--456",

journal = "Applied Surface Science",

issn = "0169-4332",

publisher = "Elsevier BV",

}

TY - JOUR

T1 - Imaging ToF-SIMS and synchrotron-based FT-IR microspectroscopic studies of prostate cancer cell lines

AU - Gazi, E.

AU - Lockyer, N. P.

AU - Vickerman, J. C.

AU - Gardner, P.

AU - Dwyer, J.

AU - Hart, C. A.

AU - Brown, M. D.

AU - Clarke, N. W.

AU - Miyan, J.

PY - 2004/6/15

Y1 - 2004/6/15

N2 - Imaging ToF-SIMS and synchrotron-based Fourier transform infrared (SR-FT-IR) microspectroscopy have been used to obtain chemical information from individual cells derived from human prostate cancer (CaP). ToF-SIMS imaging of molecular signals characteristic of membrane bound phospholipids are used to elucidate different fracture planes within individual freeze-fractured CaP cells. The localisation of Cu within the cytoplasm of cancer cells is consistent with increased metastatic potential. Line scans across CaP cells using SR-FT-IR microspectroscopy provide complimentary information on the localisation (±1μm) of lipid and protein domains. This combined analytical approach offers a novel means of characterising individual CaP cells and investigating the biochemical basis of disease progression and metastases. © 2004 Elsevier B.V. All rights reserved.

AB - Imaging ToF-SIMS and synchrotron-based Fourier transform infrared (SR-FT-IR) microspectroscopy have been used to obtain chemical information from individual cells derived from human prostate cancer (CaP). ToF-SIMS imaging of molecular signals characteristic of membrane bound phospholipids are used to elucidate different fracture planes within individual freeze-fractured CaP cells. The localisation of Cu within the cytoplasm of cancer cells is consistent with increased metastatic potential. Line scans across CaP cells using SR-FT-IR microspectroscopy provide complimentary information on the localisation (±1μm) of lipid and protein domains. This combined analytical approach offers a novel means of characterising individual CaP cells and investigating the biochemical basis of disease progression and metastases. © 2004 Elsevier B.V. All rights reserved.

KW - Cell lines

KW - FT-IR microspectroscopy

KW - Imaging

KW - Prostate cancer

KW - ToF-SIMS

U2 - 10.1016/j.apsusc.2004.03.170

DO - 10.1016/j.apsusc.2004.03.170

M3 - Article

SN - 0169-4332

VL - 231-232

SP - 452

EP - 456

JO - Applied Surface Science

JF - Applied Surface Science

ER -

Imaging ToF-SIMS and synchrotron-based FT-IR microspectroscopic studies of prostate cancer cell lines

Abstract

Keywords

UN SDGs

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