Immobilization of heparan sulfate on electrospun meshes to support embryonic stem cell culture and differentiation

Kate A. Meade; Kathryn J. White; Claire E. Pickford; Rebecca J. Holley; Andrew Marson; Donna Tillotson; Toin H. Van Kuppevelt; Jason D. Whittle; Anthony J. Day; Catherine L R Merry

doi:10.1074/jbc.M112.423012

Immobilization of heparan sulfate on electrospun meshes to support embryonic stem cell culture and differentiation

Kate A. Meade
, Kathryn J. White
, Claire E. Pickford
, Rebecca J. Holley
, Andrew Marson
, Donna Tillotson
, Toin H. Van Kuppevelt
, Jason D. Whittle
, Anthony J. Day
, Catherine L R Merry

Research output: Contribution to journal › Article › peer-review

Abstract

Background: Glycosaminoglycans influence stem cell fate but their combination with biomaterials remains to be optimized. Results: GAG bound to scaffolds presented essential sulfation epitopes and proved biologically active. Conclusion: Use of plasma polymerized allylamine proved effective in functionalizing a fibrous extracellular matrix mimic. Significance: The biomaterial has broad applicability to stem cell culture and has potential future applications in regenerative medicine. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

Original language	English
Pages (from-to)	5530-5538
Number of pages	8
Journal	Journal of Biological Chemistry
Volume	288
Issue number	8
DOIs	https://doi.org/10.1074/jbc.M112.423012
Publication status	Published - 2012

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Meade, K. A., White, K. J., Pickford, C. E., Holley, R. J., Marson, A., Tillotson, D., Van Kuppevelt, T. H., Whittle, J. D., Day, A. J., & Merry, C. L. R. (2012). Immobilization of heparan sulfate on electrospun meshes to support embryonic stem cell culture and differentiation. Journal of Biological Chemistry, 288(8), 5530-5538. https://doi.org/10.1074/jbc.M112.423012

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abstract = "Background: Glycosaminoglycans influence stem cell fate but their combination with biomaterials remains to be optimized. Results: GAG bound to scaffolds presented essential sulfation epitopes and proved biologically active. Conclusion: Use of plasma polymerized allylamine proved effective in functionalizing a fibrous extracellular matrix mimic. Significance: The biomaterial has broad applicability to stem cell culture and has potential future applications in regenerative medicine. {\textcopyright} 2013 by The American Society for Biochemistry and Molecular Biology, Inc.",

author = "Meade, \{Kate A.\} and White, \{Kathryn J.\} and Pickford, \{Claire E.\} and Holley, \{Rebecca J.\} and Andrew Marson and Donna Tillotson and \{Van Kuppevelt\}, \{Toin H.\} and Whittle, \{Jason D.\} and Day, \{Anthony J.\} and Merry, \{Catherine L R\}",

year = "2012",

doi = "10.1074/jbc.M112.423012",

language = "English",

volume = "288",

pages = "5530--5538",

journal = "Journal of Biological Chemistry",

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T1 - Immobilization of heparan sulfate on electrospun meshes to support embryonic stem cell culture and differentiation

AU - Meade, Kate A.

AU - White, Kathryn J.

AU - Pickford, Claire E.

AU - Holley, Rebecca J.

AU - Marson, Andrew

AU - Tillotson, Donna

AU - Van Kuppevelt, Toin H.

AU - Whittle, Jason D.

AU - Day, Anthony J.

AU - Merry, Catherine L R

PY - 2012

Y1 - 2012

N2 - Background: Glycosaminoglycans influence stem cell fate but their combination with biomaterials remains to be optimized. Results: GAG bound to scaffolds presented essential sulfation epitopes and proved biologically active. Conclusion: Use of plasma polymerized allylamine proved effective in functionalizing a fibrous extracellular matrix mimic. Significance: The biomaterial has broad applicability to stem cell culture and has potential future applications in regenerative medicine. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

AB - Background: Glycosaminoglycans influence stem cell fate but their combination with biomaterials remains to be optimized. Results: GAG bound to scaffolds presented essential sulfation epitopes and proved biologically active. Conclusion: Use of plasma polymerized allylamine proved effective in functionalizing a fibrous extracellular matrix mimic. Significance: The biomaterial has broad applicability to stem cell culture and has potential future applications in regenerative medicine. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

U2 - 10.1074/jbc.M112.423012

DO - 10.1074/jbc.M112.423012

M3 - Article

SN - 1083-351X

VL - 288

SP - 5530

EP - 5538

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 8

ER -

Immobilization of heparan sulfate on electrospun meshes to support embryonic stem cell culture and differentiation

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