Projects per year
While long MWCNTs dispersed in 0.5% BSA induced a granulomatous responseon the diaphragmatic mesothelium and immune cell recruitment to the peritonealcavity, GO sheets dispersed under similar conditions did not cause anyresponse, regardless of their lateral dimensions. We further interrogatedwhether tuning the surface reactivity of GO by testing different dispersions(5% dextrose instead of 0.5%BSA) may change the biological outcome. Although the change of dispersion didnot alter the impact of GO on the mesothelium (i.e. no granuloma), weobserved that, when dispersed in protein-free 5% dextrose solution, s-GOelicited a greater recruitment of monocytic cells to the peritoneal cavity thanl-GO, or when dispersed in protein containing solution. Such recruitmentcoincided with the greater ability of s-GO to interact in vivo withperitoneal macrophages and was associated with a greater surface reactivity incomparison to l-GO.
In conclusion, large dimension was not a determining factor of the immunological impact of GO sheets afteri.p. administration. For an equal dose, GO sheets with lateral dimensionssimilar to the length of long MWCNTs were less pathogenic than the MWCNTs. Onthe other hand, surface reactivity and the ability of some smaller GO sheets tointeract more readily with immune cells seem to be key parameters that can be tunedto improve the safety profile of GO. In particular, the choice of dispersionmodality, which affected these two parameters, was found to be of crucialimportance in the assessment of GO impact in this model. Overall, thesefindings are essential for a better understanding of the parameters governingGO toxicity and inflammation, and the rational design of safe GO-basedformulations for various applications, including biomedicine.
- Intraperitoneal administration
- high aspect ratio materials
- Carbon Nanotubes
- Protein coating
Research Beacons, Institutes and Platforms
- Advanced materials
- Advanced Materials in Medicine
- Lydia Becker Institute
- National Graphene Institute
FingerprintDive into the research topics of 'Immunological impact of graphene oxide sheets in the abdominal cavity is governed by surface reactivity'. Together they form a unique fingerprint.
Grigorieva, I., Burnett, H., Cusworth, E., Deaconu, D., Dumitriu-Iovanescu, A., Kang, Y., Little, J., Rees, E., Selles, F., Shaker, M., Soong, Y., Swindell, J., Tainton, G., Wood, H., Astles, T., Carl, A., Chen, G., Richard De Latour, H., Dowinton, O., Haskell, S., Hills, K., Hoole, C., Huang, Y., Kalsi, T., Powell, L., Quiligotti, K., Rimmer, J., Smith, L., Thornley, W., Yang, J., Young, W., Zhao, M., Al Busaidi, R., Al Ruqeishi, E., Chadha, A., Chen, M., Dennis, G., Dunn, E., Gamblen, E., Gao, Y., Georgantas, Y., Jiang, Z., Karakasidi, A., Mcellistrim, A., Meehan, M., Okwelogu, E., Taylor, M., Wang, W., Xin, B., Castle, C., Clout, P., Dean, S., Fordham, A., Griffin, E., Hardwick, T., Hawkins-Pottier, G., Jones, A., Lewthwaite, K., Monteil, S., Moulsdale, C., Mullan, C., Orts Mercadillo, V., Sanderson, D., Skliueva, I., Skuse, C., Steiner, P., Winstanley, B., Barry, D., Brooks, D., Cai, J., Chen, Y., Chen, C., Draude, A., Emmerson, C., Gavriliuc, V., Greaves, M., Higgins, E., Mcmaster, R., Mcnair, R., O'Brien, C., Peasey, A., Pinter, G., Shao, S., Thomas, D., Thomas, D., Tsim, L. T. B., Wengraf, J., Weston, A., Yu, T., De Libero, H., Chan, K. C., Tan, Y. T. & Thomson, T.
1/04/14 → 31/10/25
1/04/16 → 31/03/18
- 6 Article
Newman, L., Rodrigues, A. F., Jasim, D., Anna Vacchi, I., Ménard-Moyon, C., Bianco, A., Bussy, C. & Kostarelos, K., 19 Aug 2020, In: Cell Reports Physical Science. 1, 9, 100176.
Research output: Contribution to journal › Article › peer-reviewOpen Access1 Downloads (Pure)
A Blueprint for the Synthesis and Characterisation of Thin Graphene Oxide with Controlled Lateral Dimensions for BiomedicineRodrigues, A., Newman, L., Lozano Valdes, M. D. L. N., Mukherjee, S. P., Fadeel, B., Bussy, C. & Kostarelos, K., 11 May 2018, In: 2 D Materials. 5, 3
Research output: Contribution to journal › Article › peer-reviewOpen Access
Cytokine Profiling of Primary Human Macrophages Exposed to Endotoxin-Free Graphene Oxide: Size-Independent NLRP3 Inflammasome ActivationMukherjee, S. P., Kostarelos, K. & Fadeel, B., 21 Feb 2018, In: Advanced Healthcare Materials. 7, 4, 1700815.
Research output: Contribution to journal › Article › peer-review
- 1 Participating in a conference, workshop, exhibition, performance, inquiry, course etc