Impaired small-conductance Ca 2+-activated K + channel-dependent EDHF responses in Type II diabetic ZDF rats

Matthew P. Burnham, Ian T. Johnson, Arthur H. Weston

    Research output: Contribution to journalArticlepeer-review

    Abstract

    1 We have examined the relative contributions of small- and intermediate-conductance Ca 2+-activated K + channels (SK(Ca) and IK(Ca)) to the endothelium-derived hyperpolarizing factor (EDHF) pathway response in small mesenteric arteries of Zucker Diabetic Fatty (ZDF) rats, before and after the development of Type II diabetes, together with Lean controls. 2 Smooth muscle membrane potential was recorded using sharp microelectrodes in the presence of 10 μM indomethacin plus 100 μM N ω-nitro-L-arginine. SK(Ca) was selectively inhibited with 100 nM apamin, whereas IK(Ca) was blocked with 10 μM TRAM-39 (2-(2-chlorophenyl)- 2,2-diphenylacetonitrile). 3 Resting membrane potentials were similar in arteries from 17- to 20-week-old control and diabetic rats (approximately -54 mV). Responses elicited by 1 and 10 μM acetylcholine (ACh) were significantly smaller in the diabetic group (e.g. hyperpolarizations to -69.5±0.8 mV (ZDF; n=12) and -73.2±0.6 mV (Lean; n=12; P
    Original languageEnglish
    Pages (from-to)434-441
    Number of pages7
    JournalBritish Journal of Pharmacology
    Volume148
    Issue number4
    DOIs
    Publication statusPublished - 8 Jun 2006

    Keywords

    • Ca 2+-activated K + channel
    • Endothelium-derived hyperpolarizing factor (EDHF)
    • Mesenteric artery
    • Type II diabetes
    • Zucker Diabetic Fatty (ZDF) rat

    Fingerprint

    Dive into the research topics of 'Impaired small-conductance Ca 2+-activated K + channel-dependent EDHF responses in Type II diabetic ZDF rats'. Together they form a unique fingerprint.

    Cite this