In vitro expression of NGN3 identifies RAB3B as the predominant Ras-associated GTP-binding protein 3 family member in human islets

Karen Piper Hanley, Tom Hearn, Andrew Berry, Melanie J. Carvell, Ann Marie Patch, Louise J. Williams, Sarah A. Sugden, David I. Wilson, Sian Ellard, Neil A. Hanley

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Neurogenin 3 (NGN3) commits pancreatic progenitors to an islet cell fate. We have induced NGN3 expression and identified upregulation of the gene encoding the Ras-associated small molecular mass GTP-binding protein, RAB3B. RAB3B localised to the cytoplasm of human β-cells, both during the foetal period and post natally. Genes encoding alternative RAB3 proteins and RAB27A were unaltered by NGN3 expression and in human adult islets their transcripts were many fold less prevalent than those of RAB3B. The regulation of insulin exocytosis in rodent β-cells and responsiveness to incretins are reliant on Rab family members, notably Rab3a and Rab27a, but not Rab3b. Our results support an important inter-species difference in regulating insulin exocytosis where RAB3B is the most expressed isoform in human islets. © 2010 Society for Endocrinology.
    Original languageEnglish
    Pages (from-to)151-161
    Number of pages10
    JournalJournal of Endocrinology
    Volume207
    Issue number2
    DOIs
    Publication statusPublished - Nov 2010

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