In vivo magnetic resonance imaging to detect malignant melanoma in adult zebrafish

Samira Kabli, Shuning He, Herman P. Spaink, Adam Hurlstone, Ewa Snaar Jagalska, Huub J M De Groot, A. Alia

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Zebrafish cancer models are fast gaining ground in cancer research. Most tumors in zebrafish develop late in life, when fish are no longer transparent, limiting in vivo optical imaging methods. Thus, noninvasive imaging to track tumor in adult zebrafish remains challenging. In this study, we applied magnetic resonance microimaging (μMRI) to track spontaneous melanomas in stable transgenic zebrafish models expressing an RAS oncoprotein and lacking P53 (mitf:Ras::mitf:GFP X p53-/-). Tumors in live adult zebrafish were observed at various locations using a T2-weighted fast spin echo sequence at 9.4 T. Further, live imaging of tumors at ultrahigh field (17.6 T) revealed significant tumor heterogeneity. This heterogeneity was also confirmed by the significant differences in transverse relaxation time, T2 measured in various regions of tumor. To our knowledge, this is the first report demonstrating the application of μMRI to detect the locations, invasion status, and characteristics of internal melanomas in zebrafish and suggesting that noninvasive μMRI can be applied for longitudinal studies to track tumor development and real-time assessment of therapeutic effects in zebrafish tumor models. © Copyright 2010, Mary Ann Liebert, Inc.
    Original languageEnglish
    Pages (from-to)143-148
    Number of pages5
    JournalZebrafish
    Volume7
    Issue number2
    DOIs
    Publication statusPublished - 1 Jun 2010

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