Incorporation of N-heterocyclic cations into proteins with a highly directed chemical modification

Nicola McMillan; Jesus M. De La Fuente; Alexis D.C. Parenty; Nikolaj Gadegaard; Andrew R. Pitt; Katrina Thomson; Cameron MacKenzie; Sharon M. Kelly; Leroy Cronin

doi:10.1039/b702575k

Incorporation of N-heterocyclic cations into proteins with a highly directed chemical modification

Nicola McMillan, Jesus M. De La Fuente, Alexis D.C. Parenty, Nikolaj Gadegaard, Andrew R. Pitt, Katrina Thomson, Cameron MacKenzie, Sharon M. Kelly, Leroy Cronin^*

^*Corresponding author for this work

Analytical, Measurements and Physical Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

N-Heterocyclic cations are incorporated into proteins using 5-(2-bromoethyl)phenanthridinium bromide, which selectively reacts with either cysteine or lysine residues, resulting in ethylphenanthridinium (Phen) or highly stable cyclised dihydro-imidazo-phenanthridinium (DIP) adducts respectively; these modifications have been found to manipulate the observed structure of lysozyme and bovine serum albumin by AFM.

Original language	English
Pages (from-to)	2581-2583
Number of pages	3
Journal	Chemical Communications
Volume	2007
Issue number	25
DOIs	https://doi.org/10.1039/b702575k
Publication status	Published - 29 May 2007

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title = "Incorporation of N-heterocyclic cations into proteins with a highly directed chemical modification",

abstract = "N-Heterocyclic cations are incorporated into proteins using 5-(2-bromoethyl)phenanthridinium bromide, which selectively reacts with either cysteine or lysine residues, resulting in ethylphenanthridinium (Phen) or highly stable cyclised dihydro-imidazo-phenanthridinium (DIP) adducts respectively; these modifications have been found to manipulate the observed structure of lysozyme and bovine serum albumin by AFM.",

author = "Nicola McMillan and {De La Fuente}, {Jesus M.} and Parenty, {Alexis D.C.} and Nikolaj Gadegaard and Pitt, {Andrew R.} and Katrina Thomson and Cameron MacKenzie and Kelly, {Sharon M.} and Leroy Cronin",

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language = "English",

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T1 - Incorporation of N-heterocyclic cations into proteins with a highly directed chemical modification

AU - McMillan, Nicola

AU - De La Fuente, Jesus M.

AU - Parenty, Alexis D.C.

AU - Gadegaard, Nikolaj

AU - Pitt, Andrew R.

AU - Thomson, Katrina

AU - MacKenzie, Cameron

AU - Kelly, Sharon M.

AU - Cronin, Leroy

PY - 2007/5/29

Y1 - 2007/5/29

N2 - N-Heterocyclic cations are incorporated into proteins using 5-(2-bromoethyl)phenanthridinium bromide, which selectively reacts with either cysteine or lysine residues, resulting in ethylphenanthridinium (Phen) or highly stable cyclised dihydro-imidazo-phenanthridinium (DIP) adducts respectively; these modifications have been found to manipulate the observed structure of lysozyme and bovine serum albumin by AFM.

AB - N-Heterocyclic cations are incorporated into proteins using 5-(2-bromoethyl)phenanthridinium bromide, which selectively reacts with either cysteine or lysine residues, resulting in ethylphenanthridinium (Phen) or highly stable cyclised dihydro-imidazo-phenanthridinium (DIP) adducts respectively; these modifications have been found to manipulate the observed structure of lysozyme and bovine serum albumin by AFM.

UR - http://www.scopus.com/inward/record.url?scp=34250734517&partnerID=8YFLogxK

U2 - 10.1039/b702575k

DO - 10.1039/b702575k

M3 - Article

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AN - SCOPUS:34250734517

SN - 1359-7345

VL - 2007

SP - 2581

EP - 2583

JO - Chemical Communications

JF - Chemical Communications

IS - 25

ER -

Incorporation of N-heterocyclic cations into proteins with a highly directed chemical modification

Abstract

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