Increased Ca2+ sensitivity as a key mechanism of PKC-induced constriction in pressurized cerebral arteries

The effects of activating protein kinase C (PKC) with indolactam V (Indo-V) and 1,2-dioctanoyl-sn-glycerol (DOG) on smooth muscle intracellular Ca2+ concentrations ([Ca2+](i)) and arterial diameter were determined using ratiometric Ca2+ imaging and video edge detection of pressurized rat posterior cerebral arteries. Elevation of intraluminal pressure from 10 to 60 mmHg resulted in an increase in [Ca2+](i) from 74 ± 5 to 219 ± 8 nM and myogenic constriction. Application of Indo-V (0.01-3 μM) or DOG (0.1-30 μM) induced constriction and decreased [Ca2+](i) to 140 ± 11 and 127 ± 12 nM, respectively, at the highest concentrations used. In the presence of Indo-V, the dihydropyridine Ca2+-channel-blocker nisoldipine produced nearly maximum dilation and decreased [Ca2+](i) to 97 ± 7 nM. In α-toxin- permeabilized arteries, the constrictor effects of Indo-V and DOG were not observed in the absence of Ca2+. Both PKC activators significantly increased the degree of constriction of permeabilized arteries at different [Ca2+](i). We conclude that 1) Indo-V- or DOG-induced constriction of pressurized arteries requires Ca2+ influx through voltage-dependent Ca2+ channels, and 2) PKC-induced constriction of pressurized rat cerebral arteries is associated with a decrease in [Ca2+](i), suggesting an increase in the Ca2+ sensitivity of the contractile process.