Increased levels of soluble interleukin-6 receptor and CCL3 in COPD sputum.

Arjun K Ravi; Shruti Khurana; Jonathan Lemon; Jonathan Plumb; George Booth; Louise Healy; Matthew Catley; Jørgen Vestbo; Dave Singh; Sukh Singh

doi:10.1186/s12931-014-0103-4

Increased levels of soluble interleukin-6 receptor and CCL3 in COPD sputum.

Arjun K Ravi, Shruti Khurana, Jonathan Lemon, Jonathan Plumb, George Booth, Louise Healy, Matthew Catley, Jørgen Vestbo, Dave Singh, Sukh Singh

Research output: Contribution to journal › Article › peer-review

Abstract

BACKGROUND: COPD patients have increased numbers of macrophages and neutrophils in the lungs. Interleukin-6 (IL-6) trans-signaling via its soluble receptor sIL-6R, governs the influx of innate immune cells to inflammatory foci through regulation of the chemokine CCL3. We hypothesized that there would be enhanced levels of IL-6, sIL-6R and CCL3 in COPD sputum. METHODS: 59 COPD patients, 15 HNS and 15 S underwent sputum induction and processing with phosphate buffered saline to obtain supernatants for IL-6, sIL-6R and CCL3 analysis. Cytoslides were produced for differential cell counting and immunocytochemistry (COPD; n = 3) to determine cell type surface expression of the CCL3 receptors CCR5 and CCR1. RESULTS: COPD patients expressed higher levels (p

Original language	English
Article number	103
Journal	Respiratory research
Volume	15
Issue number	1
DOIs	https://doi.org/10.1186/s12931-014-0103-4
Publication status	Published - 2014

Keywords

CCL3
COPD
Interleukin-6
sIL-6R
Sputum
Two-step sputum processing

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10.1186/s12931-014-0103-4

http://respiratory-research.com/content/15/1/103

Cite this

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title = "Increased levels of soluble interleukin-6 receptor and CCL3 in COPD sputum.",

abstract = "BACKGROUND: COPD patients have increased numbers of macrophages and neutrophils in the lungs. Interleukin-6 (IL-6) trans-signaling via its soluble receptor sIL-6R, governs the influx of innate immune cells to inflammatory foci through regulation of the chemokine CCL3. We hypothesized that there would be enhanced levels of IL-6, sIL-6R and CCL3 in COPD sputum. METHODS: 59 COPD patients, 15 HNS and 15 S underwent sputum induction and processing with phosphate buffered saline to obtain supernatants for IL-6, sIL-6R and CCL3 analysis. Cytoslides were produced for differential cell counting and immunocytochemistry (COPD; n = 3) to determine cell type surface expression of the CCL3 receptors CCR5 and CCR1. RESULTS: COPD patients expressed higher levels (p ",

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year = "2014",

doi = "10.1186/s12931-014-0103-4",

language = "English",

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journal = "Respiratory research",

issn = "1465-993X",

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T1 - Increased levels of soluble interleukin-6 receptor and CCL3 in COPD sputum.

AU - Ravi, Arjun K

AU - Khurana, Shruti

AU - Lemon, Jonathan

AU - Plumb, Jonathan

AU - Booth, George

AU - Healy, Louise

AU - Catley, Matthew

AU - Vestbo, Jørgen

AU - Singh, Dave

AU - Singh, Sukh

PY - 2014

Y1 - 2014

N2 - BACKGROUND: COPD patients have increased numbers of macrophages and neutrophils in the lungs. Interleukin-6 (IL-6) trans-signaling via its soluble receptor sIL-6R, governs the influx of innate immune cells to inflammatory foci through regulation of the chemokine CCL3. We hypothesized that there would be enhanced levels of IL-6, sIL-6R and CCL3 in COPD sputum. METHODS: 59 COPD patients, 15 HNS and 15 S underwent sputum induction and processing with phosphate buffered saline to obtain supernatants for IL-6, sIL-6R and CCL3 analysis. Cytoslides were produced for differential cell counting and immunocytochemistry (COPD; n = 3) to determine cell type surface expression of the CCL3 receptors CCR5 and CCR1. RESULTS: COPD patients expressed higher levels (p

AB - BACKGROUND: COPD patients have increased numbers of macrophages and neutrophils in the lungs. Interleukin-6 (IL-6) trans-signaling via its soluble receptor sIL-6R, governs the influx of innate immune cells to inflammatory foci through regulation of the chemokine CCL3. We hypothesized that there would be enhanced levels of IL-6, sIL-6R and CCL3 in COPD sputum. METHODS: 59 COPD patients, 15 HNS and 15 S underwent sputum induction and processing with phosphate buffered saline to obtain supernatants for IL-6, sIL-6R and CCL3 analysis. Cytoslides were produced for differential cell counting and immunocytochemistry (COPD; n = 3) to determine cell type surface expression of the CCL3 receptors CCR5 and CCR1. RESULTS: COPD patients expressed higher levels (p

KW - CCL3

KW - COPD

KW - Interleukin-6

KW - sIL-6R

KW - Sputum

KW - Two-step sputum processing

U2 - 10.1186/s12931-014-0103-4

DO - 10.1186/s12931-014-0103-4

M3 - Article

C2 - 25183374

SN - 1465-993X

VL - 15

JO - Respiratory research

JF - Respiratory research

IS - 1

M1 - 103

ER -

Increased levels of soluble interleukin-6 receptor and CCL3 in COPD sputum.

Abstract

Keywords

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