IncucyteDRC: An R package for the dose response analysis of live cell imaging data

Philip J Chapman; Dominic I James; Amanda J Watson; Gemma V Hopkins; Ian D Waddell; Donald J Ogilvie

doi:10.12688/f1000research.8694.1

IncucyteDRC: An R package for the dose response analysis of live cell imaging data

Philip J Chapman, Dominic I James, Amanda J Watson, Gemma V Hopkins, Ian D Waddell, Donald J Ogilvie

Cancer Research UK Manchester Institute

NIHR Manchester Biomedical Research Centre

Research output: Contribution to journal › Article › peer-review

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Abstract

We present IncucyteDRC, an R package for the analysis of data from live cell imaging cell proliferation experiments carried out on the Essen Biosciences IncuCyte ZOOM instrument. The package provides a simple workflow for summarising data into a form that can be used to calculate dose response curves and EC50 values for small molecule inhibitors. Data from different cell lines, or cell lines grown under different conditions, can be normalised as to their doubling time. A simple graphical web interface, implemented using shiny, is provided for the benefit of non-R users. The software is potentially useful to any research group studying the impact of small molecule inhibitors on cell proliferation using the IncuCyte ZOOM.

Original language	English
Pages (from-to)	962
Journal	F1000Research
Volume	5
Issue number	962
DOIs	https://doi.org/10.12688/f1000research.8694.1
Publication status	Published - 23 May 2016

Keywords

R, Incycyte

Research Beacons, Institutes and Platforms

Manchester Cancer Research Centre

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "IncucyteDRC: An R package for the dose response analysis of live cell imaging data",

abstract = "We present IncucyteDRC, an R package for the analysis of data from live cell imaging cell proliferation experiments carried out on the Essen Biosciences IncuCyte ZOOM instrument. The package provides a simple workflow for summarising data into a form that can be used to calculate dose response curves and EC50 values for small molecule inhibitors. Data from different cell lines, or cell lines grown under different conditions, can be normalised as to their doubling time. A simple graphical web interface, implemented using shiny, is provided for the benefit of non-R users. The software is potentially useful to any research group studying the impact of small molecule inhibitors on cell proliferation using the IncuCyte ZOOM.",

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AU - Watson, Amanda J

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AU - Waddell, Ian D

AU - Ogilvie, Donald J

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IncucyteDRC: An R package for the dose response analysis of live cell imaging data

Abstract

Keywords

Research Beacons, Institutes and Platforms

UN SDGs

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