TY - JOUR
T1 - Inducible nitric oxide synthase promoter polymorphism in human brucellosis
AU - Orozco, Gisela
AU - Sánchez, Elena
AU - López-Nevot, Miguel Angel
AU - Caballero, Abelardo
AU - Bravo, María José
AU - Morata, Pilar
AU - De Dios Colmenero, Juan
AU - Alonso, Antonio
AU - Martín, Javier
PY - 2003/11
Y1 - 2003/11
N2 - Nitric oxide (NO), produced by the inducible nitric oxide synthase (NOS2) protein, is a defence mechanism against various pathogens, including bacteria of the genus Brucella. The aim of this study was to investigate the possible association between the NOS2 gene promoter polymorphism, TAAAn and CCTTTn microsatellites, and the predisposition to human brucellosis. We performed a case-control study in 85 patients with brucellosis and 100 healthy individuals, matched for age and sex, living in the same geographic area, in whom the NOS2 promoter was genotyped by using a polymerase chain reaction (PCR)-based method combined with fluorescent technology. No statistically significant differences were observed in the distribution of TAAAn alleles between the groups under study. When the overall NOS2 CCTTTn allele distribution of the brucellosis patients was compared with that of the control subjects, a significant skewing was observed (P = 0.04, by χ2 test from 2 × 9 contingency table). Interestingly, we observed a trend towards Brucella infection protection with the 9 repeat (181 bp) allele (1.8% patients vs. 7.5% controls; P = 0.01, odds ratios = 0.22, 95% confidence interval = 0.05-0.83), which turned out to be non-significant after applying multiple testing. We concluded that the NOS2 microsatellite polymorphism might not have a major effect on brucellosis; nevertheless, the fact that a non-significant trend towards protection was detected in the CCTTTn alleles may be an indication for a follow-up study. © 2003 Éditions scientifiques et médicales Elsevier SAS. All rights reserved.
AB - Nitric oxide (NO), produced by the inducible nitric oxide synthase (NOS2) protein, is a defence mechanism against various pathogens, including bacteria of the genus Brucella. The aim of this study was to investigate the possible association between the NOS2 gene promoter polymorphism, TAAAn and CCTTTn microsatellites, and the predisposition to human brucellosis. We performed a case-control study in 85 patients with brucellosis and 100 healthy individuals, matched for age and sex, living in the same geographic area, in whom the NOS2 promoter was genotyped by using a polymerase chain reaction (PCR)-based method combined with fluorescent technology. No statistically significant differences were observed in the distribution of TAAAn alleles between the groups under study. When the overall NOS2 CCTTTn allele distribution of the brucellosis patients was compared with that of the control subjects, a significant skewing was observed (P = 0.04, by χ2 test from 2 × 9 contingency table). Interestingly, we observed a trend towards Brucella infection protection with the 9 repeat (181 bp) allele (1.8% patients vs. 7.5% controls; P = 0.01, odds ratios = 0.22, 95% confidence interval = 0.05-0.83), which turned out to be non-significant after applying multiple testing. We concluded that the NOS2 microsatellite polymorphism might not have a major effect on brucellosis; nevertheless, the fact that a non-significant trend towards protection was detected in the CCTTTn alleles may be an indication for a follow-up study. © 2003 Éditions scientifiques et médicales Elsevier SAS. All rights reserved.
KW - Human brucellosis
KW - Microsatellite polymorphism
KW - Nitric oxide synthase
U2 - 10.1016/j.micinf.2003.08.010
DO - 10.1016/j.micinf.2003.08.010
M3 - Article
SN - 1286-4579
VL - 5
SP - 1165
EP - 1169
JO - Microbes and Infection
JF - Microbes and Infection
IS - 13
ER -