Induction of Egr-1 mRNA and protein by endothelin 1, angiotensin II and norepinephrine in neonatal cardiac myocytes

Asiya Shamim, Theo Pelzer, Christian Grohé, Ludwig Neyses

    Research output: Contribution to journalArticlepeer-review


    The early growth response gene Egr-1 is a nuclear transcription factor known to serve as an intermediary in a broad range of signal transduction processes. Recent studies have assigned Egr-1 a new role as an amplifier of gene expression. Egr-1 mRNA is expressed in the myocardium and is rapidly induced in response to hypertrophic stimuli. However, induction of the Egr-1 protein has not yet been demonstrated in the myocardium; on the other hand, in skeletal muscle cells we have shown translational regulation of Egr-1. To further investigate the role of Egr-1 in the regulatory mechanisms of a variety of signal transduction processes we have therefore asked whether bona fide hypertrophic stimuli induce Egr-1 protein subsequently to its mRNA in neonatal rat cardiomyocytes or whether translational block occurs. In confocal laser studies the Egr-1 protein was nuclearly localized. Norepinephrine (NE, 2 μM), angiotensin II (AII, 0.1 μM), and endothelin 1 (E1, 0.1 μM) each induced the Egr-1 mRNA 6-8 fold and the Egr-1 protein 3-5 fold (n = 3, p <0.01). Therefore, in contrast to skeletal muscle cells, these stimuli increased Egr-1 mRNA ann protein levels. These results point further to the role of Egr-1 as a possible amplifier of signal transduction in the myocardium.
    Original languageEnglish
    Pages (from-to)11-17
    Number of pages6
    JournalMolecular and Cellular Biochemistry
    Issue number1-2
    Publication statusPublished - 1999


    • Angiotensin II
    • Early growth response gene
    • Endothelin 1
    • Neonatal cardiomyocytes
    • Norepinephrine


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