Inhibition of aldose reductase: 13C NMR studies in isolated peripheral nerve.

J. C. Waterton, S. A. Breen, D. J. Mirrlees, C. M. Sennitt, F. Carey

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    We report 13C NMR measurements of the flux through aldose reductase in isolated rat sciatic nerve, and its inhibition by an aldose reductase inhibitor of the sulphonylnitromethane class. [1-13C] galactose was used as substrate, and the rate of production of [1-13C] dulcitol was measured. Quantitation required the use both of internal extracellular, and external, standards. The mean net forward flux (+/- SD) was 20 +/- 11 nmol/(mL nerve water)/min (n = 10). In the presence of the inhibitor, flux was reduced significantly (p <0.001) to 13% of control. Since dulcitol is symmetrical, an estimate of the backward flux, to [6-13C] galactose, is also possible; under our conditions, this was negligible.
    Original languageEnglish
    Pages (from-to)133-138
    Number of pages5
    JournalNMR in Biomedicine
    Issue number3
    Publication statusPublished - May 1995


    • antagonists & inhibitors: Aldehyde Reductase
    • Animals
    • Carbon Isotopes
    • pharmacology: Enzyme Inhibitors
    • analysis: Galactitol
    • metabolism: Galactose
    • Magnetic Resonance Spectroscopy
    • Male
    • pharmacology: Nitroparaffins
    • Rats
    • Rats, Wistar
    • enzymology: Sciatic Nerve
    • pharmacology: Sulfones


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