InsP3-induced Ca2+ release in dorsal root ganglion neurones

Natalia Svichar; Anatoly Shmigol; Alexej Verkhratsky; Platon Kostyuk

doi:10.1016/S0304-3940(97)00313-3

InsP3-induced Ca2+ release in dorsal root ganglion neurones

Natalia Svichar, Anatoly Shmigol, Alexej Verkhratsky, Platon Kostyuk

Research output: Contribution to journal › Article › peer-review

Abstract

The intracellular calcium signaling was studied on subpopulation of freshly isolated adult mouse dorsal root ganglia (DRG) neurones with large somatas (30-45 μ in diameter). The cytoplasmic Ca2+ concentration ([Ca2+](i)) was measured using indo-1 based microfluorimetry. The extracellular application of ATP (100 μM) triggered both inward current and [Ca2+](i) elevation. Removal of extracellular Ca2+ had no effect on both ATP-induced current and [Ca2+](i) transient. The ATP-induced Ca2+ elevation was inhibited by intracellular perfusion of DRG neurones with 20 μM heparin, or by cells incubation with thapsigargin or ryanodine. We conclude that mouse proprioceptive sensory neurones are endowed with Ca2+ - impermeable ionotropic P(2X) purinoreceptors and metabotropic P(2Y) purinoreceptors, which, by means of phospholipase C-drive inositol- trisphosphate (InsP3) production, trigger the InsP3-induced Ca2+ release from intracellular stores.

Original language	English
Pages (from-to)	107-110
Number of pages	3
Journal	Neuroscience letters
Volume	227
Issue number	2
DOIs	https://doi.org/10.1016/S0304-3940(97)00313-3
Publication status	Published - 16 May 1997

Keywords

Ca2+ release
Caffeine
Cytoplasmic calcium
Inositol-trisphosphate (InsP3)
Purinoreceptors
Sensory neurones

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10.1016/S0304-3940(97)00313-3

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title = "InsP3-induced Ca2+ release in dorsal root ganglion neurones",

abstract = "The intracellular calcium signaling was studied on subpopulation of freshly isolated adult mouse dorsal root ganglia (DRG) neurones with large somatas (30-45 μ in diameter). The cytoplasmic Ca2+ concentration ([Ca2+](i)) was measured using indo-1 based microfluorimetry. The extracellular application of ATP (100 μM) triggered both inward current and [Ca2+](i) elevation. Removal of extracellular Ca2+ had no effect on both ATP-induced current and [Ca2+](i) transient. The ATP-induced Ca2+ elevation was inhibited by intracellular perfusion of DRG neurones with 20 μM heparin, or by cells incubation with thapsigargin or ryanodine. We conclude that mouse proprioceptive sensory neurones are endowed with Ca2+ - impermeable ionotropic P(2X) purinoreceptors and metabotropic P(2Y) purinoreceptors, which, by means of phospholipase C-drive inositol- trisphosphate (InsP3) production, trigger the InsP3-induced Ca2+ release from intracellular stores.",

keywords = "Ca2+ release, Caffeine, Cytoplasmic calcium, Inositol-trisphosphate (InsP3), Purinoreceptors, Sensory neurones",

author = "Natalia Svichar and Anatoly Shmigol and Alexej Verkhratsky and Platon Kostyuk",

year = "1997",

month = may,

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doi = "10.1016/S0304-3940(97)00313-3",

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T1 - InsP3-induced Ca2+ release in dorsal root ganglion neurones

AU - Svichar, Natalia

AU - Shmigol, Anatoly

AU - Verkhratsky, Alexej

AU - Kostyuk, Platon

PY - 1997/5/16

Y1 - 1997/5/16

N2 - The intracellular calcium signaling was studied on subpopulation of freshly isolated adult mouse dorsal root ganglia (DRG) neurones with large somatas (30-45 μ in diameter). The cytoplasmic Ca2+ concentration ([Ca2+](i)) was measured using indo-1 based microfluorimetry. The extracellular application of ATP (100 μM) triggered both inward current and [Ca2+](i) elevation. Removal of extracellular Ca2+ had no effect on both ATP-induced current and [Ca2+](i) transient. The ATP-induced Ca2+ elevation was inhibited by intracellular perfusion of DRG neurones with 20 μM heparin, or by cells incubation with thapsigargin or ryanodine. We conclude that mouse proprioceptive sensory neurones are endowed with Ca2+ - impermeable ionotropic P(2X) purinoreceptors and metabotropic P(2Y) purinoreceptors, which, by means of phospholipase C-drive inositol- trisphosphate (InsP3) production, trigger the InsP3-induced Ca2+ release from intracellular stores.

AB - The intracellular calcium signaling was studied on subpopulation of freshly isolated adult mouse dorsal root ganglia (DRG) neurones with large somatas (30-45 μ in diameter). The cytoplasmic Ca2+ concentration ([Ca2+](i)) was measured using indo-1 based microfluorimetry. The extracellular application of ATP (100 μM) triggered both inward current and [Ca2+](i) elevation. Removal of extracellular Ca2+ had no effect on both ATP-induced current and [Ca2+](i) transient. The ATP-induced Ca2+ elevation was inhibited by intracellular perfusion of DRG neurones with 20 μM heparin, or by cells incubation with thapsigargin or ryanodine. We conclude that mouse proprioceptive sensory neurones are endowed with Ca2+ - impermeable ionotropic P(2X) purinoreceptors and metabotropic P(2Y) purinoreceptors, which, by means of phospholipase C-drive inositol- trisphosphate (InsP3) production, trigger the InsP3-induced Ca2+ release from intracellular stores.

KW - Ca2+ release

KW - Caffeine

KW - Cytoplasmic calcium

KW - Inositol-trisphosphate (InsP3)

KW - Purinoreceptors

KW - Sensory neurones

U2 - 10.1016/S0304-3940(97)00313-3

DO - 10.1016/S0304-3940(97)00313-3

M3 - Article

SN - 0304-3940

VL - 227

SP - 107

EP - 110

JO - Neuroscience letters

JF - Neuroscience letters

IS - 2

ER -

InsP3-induced Ca2+ release in dorsal root ganglion neurones

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