Insulinlike growth factor gene expression in human fracture callus

J. G. Andrew; J. Hoyland; A. J. Freemont; D. Marsh

doi:10.1007/BF01321886

Insulinlike growth factor gene expression in human fracture callus

J. G. Andrew, J. Hoyland, A. J. Freemont, D. Marsh

Research output: Contribution to journal › Article › peer-review

Abstract

The effects of insulinlike growth factors on bone and cartilage-derived cells in culture have been extensively investigated, but there is little information on their role in vivo in bone, especially in fracture healing. This study investigated insulinlike growth factor (IGF) I and II mRNA expression in normally healing human fractures by in situ hybridization. Endothelial and mesenchymal cells at the granulation tissue stage expressed IGF-II mRNA. At the stage of bone and cartilage formation, osteoblasts and nonhypertrophic chondrocytes expressed mRNA for both IGF-I and II. Some osteoclasts were positive for IGF-II mRNA at the stage of bone remodeling. The greater time span of IGF-II expression relative to IGF-I reflects the predominance of IGF-II in human bone matrix. Taken together with the known effects of IGFs on bone and cartilage cells in vitro, these findings support a role for IGFs in local cellular regulation in human fracture healing. © 1993 Springer-Verlag New York Inc.

Original language	English
Pages (from-to)	97-102
Number of pages	5
Journal	Calcified tissue international
Volume	53
Issue number	2
DOIs	https://doi.org/10.1007/BF01321886
Publication status	Published - Aug 1993

Keywords

Fracture
In situ hybridization
Insulinlike growth factor

Access to Document

10.1007/BF01321886

Cite this

@article{b5c9d7064ee14d758509dbb8d7e9bf64,

title = "Insulinlike growth factor gene expression in human fracture callus",

abstract = "The effects of insulinlike growth factors on bone and cartilage-derived cells in culture have been extensively investigated, but there is little information on their role in vivo in bone, especially in fracture healing. This study investigated insulinlike growth factor (IGF) I and II mRNA expression in normally healing human fractures by in situ hybridization. Endothelial and mesenchymal cells at the granulation tissue stage expressed IGF-II mRNA. At the stage of bone and cartilage formation, osteoblasts and nonhypertrophic chondrocytes expressed mRNA for both IGF-I and II. Some osteoclasts were positive for IGF-II mRNA at the stage of bone remodeling. The greater time span of IGF-II expression relative to IGF-I reflects the predominance of IGF-II in human bone matrix. Taken together with the known effects of IGFs on bone and cartilage cells in vitro, these findings support a role for IGFs in local cellular regulation in human fracture healing. {\textcopyright} 1993 Springer-Verlag New York Inc.",

keywords = "Fracture, In situ hybridization, Insulinlike growth factor",

author = "Andrew, {J. G.} and J. Hoyland and Freemont, {A. J.} and D. Marsh",

year = "1993",

month = aug,

doi = "10.1007/BF01321886",

language = "English",

volume = "53",

pages = "97--102",

journal = "Calcified tissue international",

issn = "1432-0827",

publisher = "Springer Nature",

number = "2",

}

TY - JOUR

T1 - Insulinlike growth factor gene expression in human fracture callus

AU - Andrew, J. G.

AU - Hoyland, J.

AU - Freemont, A. J.

AU - Marsh, D.

PY - 1993/8

Y1 - 1993/8

N2 - The effects of insulinlike growth factors on bone and cartilage-derived cells in culture have been extensively investigated, but there is little information on their role in vivo in bone, especially in fracture healing. This study investigated insulinlike growth factor (IGF) I and II mRNA expression in normally healing human fractures by in situ hybridization. Endothelial and mesenchymal cells at the granulation tissue stage expressed IGF-II mRNA. At the stage of bone and cartilage formation, osteoblasts and nonhypertrophic chondrocytes expressed mRNA for both IGF-I and II. Some osteoclasts were positive for IGF-II mRNA at the stage of bone remodeling. The greater time span of IGF-II expression relative to IGF-I reflects the predominance of IGF-II in human bone matrix. Taken together with the known effects of IGFs on bone and cartilage cells in vitro, these findings support a role for IGFs in local cellular regulation in human fracture healing. © 1993 Springer-Verlag New York Inc.

AB - The effects of insulinlike growth factors on bone and cartilage-derived cells in culture have been extensively investigated, but there is little information on their role in vivo in bone, especially in fracture healing. This study investigated insulinlike growth factor (IGF) I and II mRNA expression in normally healing human fractures by in situ hybridization. Endothelial and mesenchymal cells at the granulation tissue stage expressed IGF-II mRNA. At the stage of bone and cartilage formation, osteoblasts and nonhypertrophic chondrocytes expressed mRNA for both IGF-I and II. Some osteoclasts were positive for IGF-II mRNA at the stage of bone remodeling. The greater time span of IGF-II expression relative to IGF-I reflects the predominance of IGF-II in human bone matrix. Taken together with the known effects of IGFs on bone and cartilage cells in vitro, these findings support a role for IGFs in local cellular regulation in human fracture healing. © 1993 Springer-Verlag New York Inc.

KW - Fracture

KW - In situ hybridization

KW - Insulinlike growth factor

U2 - 10.1007/BF01321886

DO - 10.1007/BF01321886

M3 - Article

SN - 1432-0827

VL - 53

SP - 97

EP - 102

JO - Calcified tissue international

JF - Calcified tissue international

IS - 2

ER -

Insulinlike growth factor gene expression in human fracture callus

Abstract

Keywords

Access to Document

Fingerprint

Cite this