Integrating Cell Sheets for Organ-on-a-chip Applications

Research output: Contribution to journalConference articlepeer-review

Abstract

To improve on attrition rates during drug development due to nephrotoxicity, a better in vitro model of the kidney is needed. We demonstrate a model of the kidney proximal tubule in which a sheet of proximal tubule epithelial cells is fixed as a membrane separating two fluid chambers without the presence of a supporting membrane. Cell sheets were prepared using a Poly(N-isopropylacrylamide) (PIPAAm) temperature responsive surface and a bespoke bioreactor was constructed using a 3D printer to produce an appropriate mould. Cell sheets were suspended using a fibronectin functionalized surface seeded with cells. Further testing is needed to determine viability in bioreactor.

Original languageEnglish
Pages (from-to)127-130
Number of pages4
JournalProcedia CIRP
Volume65
Early online date21 Jul 2017
DOIs
Publication statusPublished - 2017
Event3rd CIRP Conference on BioManufacturing 2017 - Chicago, United States
Duration: 11 Jul 201714 Jul 2017

Keywords

  • bioreactor
  • cell sheet engineering
  • kidney
  • microfluidics
  • organ on a chip
  • PDMS
  • PIPAAm
  • temperature responsive

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