Integrating Cell Sheets for Organ-on-a-chip Applications

William Loewenhardt; Rachel Saunders; Rachel Lennon; Brian Derby

doi:10.1016/j.procir.2017.04.018

Integrating Cell Sheets for Organ-on-a-chip Applications

William Loewenhardt, Rachel Saunders, Rachel Lennon, Brian Derby^*

^*Corresponding author for this work

Research output: Contribution to journal › Conference article › peer-review

Abstract

To improve on attrition rates during drug development due to nephrotoxicity, a better in vitro model of the kidney is needed. We demonstrate a model of the kidney proximal tubule in which a sheet of proximal tubule epithelial cells is fixed as a membrane separating two fluid chambers without the presence of a supporting membrane. Cell sheets were prepared using a Poly(N-isopropylacrylamide) (PIPAAm) temperature responsive surface and a bespoke bioreactor was constructed using a 3D printer to produce an appropriate mould. Cell sheets were suspended using a fibronectin functionalized surface seeded with cells. Further testing is needed to determine viability in bioreactor.

Original language	English
Pages (from-to)	127-130
Number of pages	4
Journal	Procedia CIRP
Volume	65
Early online date	21 Jul 2017
DOIs	https://doi.org/10.1016/j.procir.2017.04.018
Publication status	Published - 2017
Event	3rd CIRP Conference on BioManufacturing 2017 - Chicago, United States Duration: 11 Jul 2017 → 14 Jul 2017

Keywords

bioreactor
cell sheet engineering
kidney
microfluidics
organ on a chip
PDMS
PIPAAm
temperature responsive

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10.1016/j.procir.2017.04.018

Advanced Materials & Development
Saunders, R. (Senior Technical Specialist), Wallwork, A. (Senior Technical Specialist), Hassan-Naji, R. (Senior Technician) & Wallwork, O. (Technician)
Materials - Administration
Facility/equipment: Platform

Cite this

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title = "Integrating Cell Sheets for Organ-on-a-chip Applications",

abstract = "To improve on attrition rates during drug development due to nephrotoxicity, a better in vitro model of the kidney is needed. We demonstrate a model of the kidney proximal tubule in which a sheet of proximal tubule epithelial cells is fixed as a membrane separating two fluid chambers without the presence of a supporting membrane. Cell sheets were prepared using a Poly(N-isopropylacrylamide) (PIPAAm) temperature responsive surface and a bespoke bioreactor was constructed using a 3D printer to produce an appropriate mould. Cell sheets were suspended using a fibronectin functionalized surface seeded with cells. Further testing is needed to determine viability in bioreactor.",

keywords = "bioreactor, cell sheet engineering, kidney, microfluidics, organ on a chip, PDMS, PIPAAm, temperature responsive",

author = "William Loewenhardt and Rachel Saunders and Rachel Lennon and Brian Derby",

year = "2017",

doi = "10.1016/j.procir.2017.04.018",

language = "English",

volume = "65",

pages = "127--130",

journal = "Procedia CIRP",

issn = "2212-8271",

publisher = "Elsevier BV",

note = "3rd CIRP Conference on BioManufacturing 2017 ; Conference date: 11-07-2017 Through 14-07-2017",

}

TY - JOUR

T1 - Integrating Cell Sheets for Organ-on-a-chip Applications

AU - Loewenhardt, William

AU - Saunders, Rachel

AU - Lennon, Rachel

AU - Derby, Brian

PY - 2017

Y1 - 2017

N2 - To improve on attrition rates during drug development due to nephrotoxicity, a better in vitro model of the kidney is needed. We demonstrate a model of the kidney proximal tubule in which a sheet of proximal tubule epithelial cells is fixed as a membrane separating two fluid chambers without the presence of a supporting membrane. Cell sheets were prepared using a Poly(N-isopropylacrylamide) (PIPAAm) temperature responsive surface and a bespoke bioreactor was constructed using a 3D printer to produce an appropriate mould. Cell sheets were suspended using a fibronectin functionalized surface seeded with cells. Further testing is needed to determine viability in bioreactor.

AB - To improve on attrition rates during drug development due to nephrotoxicity, a better in vitro model of the kidney is needed. We demonstrate a model of the kidney proximal tubule in which a sheet of proximal tubule epithelial cells is fixed as a membrane separating two fluid chambers without the presence of a supporting membrane. Cell sheets were prepared using a Poly(N-isopropylacrylamide) (PIPAAm) temperature responsive surface and a bespoke bioreactor was constructed using a 3D printer to produce an appropriate mould. Cell sheets were suspended using a fibronectin functionalized surface seeded with cells. Further testing is needed to determine viability in bioreactor.

KW - bioreactor

KW - cell sheet engineering

KW - kidney

KW - microfluidics

KW - organ on a chip

KW - PDMS

KW - PIPAAm

KW - temperature responsive

U2 - 10.1016/j.procir.2017.04.018

DO - 10.1016/j.procir.2017.04.018

M3 - Conference article

AN - SCOPUS:85029679222

SN - 2212-8271

VL - 65

SP - 127

EP - 130

JO - Procedia CIRP

JF - Procedia CIRP

T2 - 3rd CIRP Conference on BioManufacturing 2017

Y2 - 11 July 2017 through 14 July 2017

ER -

Integrating Cell Sheets for Organ-on-a-chip Applications

Abstract

Keywords

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Advanced Materials & Development

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