@article{f7eca7377d154c389d153766498b64fc,
title = "Integrative Analysis Unveils the Correlation of Aminoacyl-tRNA Biosynthesis Metabolites with the Methylation of the SEPSECS Gene in Huntington{\textquoteright}s Disease Brain Tissue",
abstract = "The impact of environmental factors on epigenetic changes is well established, and cellular function is determined not only by the genome but also by interacting partners such as metabolites. Given the significant impact of metabolism on disease progression, exploring the interaction between the metabolome and epigenome may offer new insights into Huntington{\textquoteright}s disease (HD) diagnosis and treatment. Using fourteen post-mortem HD cases and fourteen control subjects, we performed metabolomic profiling of human postmortem brain tissue (striatum and frontal lobe), and we performed DNA methylome profiling using the same frontal lobe tissue. Along with finding several perturbed metabolites and differentially methylated loci, Aminoacyl-tRNA biosynthesis (adj p-value = 0.0098) was the most significantly perturbed metabolic pathway with which two CpGs of the SEPSECS gene were correlated. This study improves our understanding of molecular biomarker connections and, importantly, increases our knowledge of metabolic alterations driving HD progression.",
keywords = "Huntington{\textquoteright}s disease, brain, epi-metabolomics, epigenetics, integrative omics, metabolomics",
author = "Sangeetha Vishweswaraiah and Ali Yilmaz and Nazia Saiyed and Abdullah Khalid and Purvesh Koladiya and Xiaobei Pan and Shirin Macias and Andrew Robinson and David Mann and Green, {Brian D} and Ieva Kerseviciute and Juozas Gordevicius and Uppala Radhakrishna and Graham, {Stewart F}",
note = "Funding Information: This work was partly funded by the generous contribution made by the Fred A. and Barbara M. Erb Foundation. We thank Biocrates (Innsbruck, Austria) for providing the AbsoluteIDQ p180 kit for the analysis of these specimens. We also acknowledge the assistance of Muhammad Bin Nasaruddin from Queen{\textquoteright}s University, Belfast for meticulously milling the post-mortem tissue for analysis. Tissue samples were supplied by The Manchester Brain Bank, which is part of the Brains for Dementia Research program, jointly funded by Alzheimer{\textquoteright}s Research UK and Alzheimer{\textquoteright}s Society. Publisher Copyright: {\textcopyright} 2023 by the authors.",
year = "2023",
month = sep,
day = "2",
doi = "10.3390/genes14091752",
language = "English",
volume = "14",
journal = "Genes",
issn = "2073-4425",
publisher = "MDPI",
number = "9",
}
