Interaction between an acidic activator and transcription factor TFIIB is required for transcriptional activation

Stefan G E Roberts, Ilho Ha, Edio Maldonado, Danny Reinberg, Michael R. Green

    Research output: Contribution to journalArticlepeer-review

    Abstract

    HOW eukaryotic promoter-specific activator proteins (activators) stimulate transcription is a central question. We have previously shown that an acidic activator can directly interact with the general transcription factor TFIIB and increase its stable assembly into a preinitiation complex1,2. We have proposed that this increase in TFIIB assembly is at least part of the mechanism by which an acidic activator functions1,2. A prediction of this hypothesis is that a TFIIB mutant unable to interact with an acidic activator could not support activated transcription, and here we present experiments that verify this prediction. In conjunction with previous studies, our results argue that interaction between an acidic activator and TFIIB is necessary for transcriptional activation.
    Original languageEnglish
    Pages (from-to)741-744
    Number of pages3
    JournalNature
    Volume363
    Issue number6431
    DOIs
    Publication statusPublished - 24 Jun 1993

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