Interfacing functionalized carbon nanohorns with primary phagocytic cells

Stéphanie Lacotte; Ainara García; Marion Décossas; Wafa T. Al-Jamal; Shouping Li; Kostas Kostarelos; Sylviane Muller; Maurizio Prato; Hélène Dumortier; Alberto Bianco

doi:10.1002/adma.200702753

Interfacing functionalized carbon nanohorns with primary phagocytic cells

Stéphanie Lacotte, Ainara García, Marion Décossas, Wafa T. Al-Jamal, Shouping Li, Kostas Kostarelos, Sylviane Muller, Maurizio Prato, Hélène Dumortier, Alberto Bianco

Research output: Contribution to journal › Article › peer-review

Abstract

The biocompatibility of functionalized carbon nanohorns (f-CNH) was analyzed regarding primary phagocytic cells. Fluorescein isothiocyanate-labeled (FITC) f-CNHs were incubated with primary peritoneal mouse macrophages for different periods of time to analyze whether macrophages can sense and internalize f-CNHs. It was found using confocal microscopy that f-CNHs were not detected in all the cells after 24 hours of incubation. The investigation revealed that f-CNHs can be obtained by a direct addition to pristine CNHs of a diamine moiety that can be further modified through a fluorescent probe, resulting in biocompatible f-CNHs. The results show that the delivery of biologically active agents using f-CNHs that internalize into cells and promote contemporarily the activation of the immune system, is a very encouraging concept.

Original language	English
Pages (from-to)	2421-2426
Number of pages	5
Journal	Advanced Materials
Volume	20
Issue number	12
DOIs	https://doi.org/10.1002/adma.200702753
Publication status	Published - 18 Jun 2008

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title = "Interfacing functionalized carbon nanohorns with primary phagocytic cells",

abstract = "The biocompatibility of functionalized carbon nanohorns (f-CNH) was analyzed regarding primary phagocytic cells. Fluorescein isothiocyanate-labeled (FITC) f-CNHs were incubated with primary peritoneal mouse macrophages for different periods of time to analyze whether macrophages can sense and internalize f-CNHs. It was found using confocal microscopy that f-CNHs were not detected in all the cells after 24 hours of incubation. The investigation revealed that f-CNHs can be obtained by a direct addition to pristine CNHs of a diamine moiety that can be further modified through a fluorescent probe, resulting in biocompatible f-CNHs. The results show that the delivery of biologically active agents using f-CNHs that internalize into cells and promote contemporarily the activation of the immune system, is a very encouraging concept.",

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doi = "10.1002/adma.200702753",

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T1 - Interfacing functionalized carbon nanohorns with primary phagocytic cells

AU - Lacotte, Stéphanie

AU - García, Ainara

AU - Décossas, Marion

AU - Al-Jamal, Wafa T.

AU - Li, Shouping

AU - Kostarelos, Kostas

AU - Muller, Sylviane

AU - Prato, Maurizio

AU - Dumortier, Hélène

AU - Bianco, Alberto

N1 - Times Cited: 16 0 16

PY - 2008/6/18

Y1 - 2008/6/18

N2 - The biocompatibility of functionalized carbon nanohorns (f-CNH) was analyzed regarding primary phagocytic cells. Fluorescein isothiocyanate-labeled (FITC) f-CNHs were incubated with primary peritoneal mouse macrophages for different periods of time to analyze whether macrophages can sense and internalize f-CNHs. It was found using confocal microscopy that f-CNHs were not detected in all the cells after 24 hours of incubation. The investigation revealed that f-CNHs can be obtained by a direct addition to pristine CNHs of a diamine moiety that can be further modified through a fluorescent probe, resulting in biocompatible f-CNHs. The results show that the delivery of biologically active agents using f-CNHs that internalize into cells and promote contemporarily the activation of the immune system, is a very encouraging concept.

AB - The biocompatibility of functionalized carbon nanohorns (f-CNH) was analyzed regarding primary phagocytic cells. Fluorescein isothiocyanate-labeled (FITC) f-CNHs were incubated with primary peritoneal mouse macrophages for different periods of time to analyze whether macrophages can sense and internalize f-CNHs. It was found using confocal microscopy that f-CNHs were not detected in all the cells after 24 hours of incubation. The investigation revealed that f-CNHs can be obtained by a direct addition to pristine CNHs of a diamine moiety that can be further modified through a fluorescent probe, resulting in biocompatible f-CNHs. The results show that the delivery of biologically active agents using f-CNHs that internalize into cells and promote contemporarily the activation of the immune system, is a very encouraging concept.

U2 - 10.1002/adma.200702753

DO - 10.1002/adma.200702753

M3 - Article

VL - 20

SP - 2421

EP - 2426

JO - Advanced Materials

JF - Advanced Materials

SN - 0935-9648

IS - 12

ER -

Interfacing functionalized carbon nanohorns with primary phagocytic cells

Abstract

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