Interleukin-1β isolated from a marine fish reveals up-regulated expression in macrophages following activation with lipopolysaccharide and lymphokines

Pablo Pelegrín; Jesús García-Castillo; Victoriano Mulero; José Meseguer

doi:10.1006/cyto.2001.0949

Interleukin-1β isolated from a marine fish reveals up-regulated expression in macrophages following activation with lipopolysaccharide and lymphokines

Pablo Pelegrín, Jesús García-Castillo, Victoriano Mulero, José Meseguer

Research output: Contribution to journal › Article › peer-review

Abstract

The gilthead seabream IL-1β gene consists of five exons/four introns. The complete coding sequence contains a 102 bp 5′ untranslated region (UTR), a single open reading frame of 762 bp which translates into a 253 amino acid molecule, and a 407 bp 3′UTR with a polyadenylation signal 14 nucleotides upstream of the poly(A)tail. The seabream sequence has the highest degree of nucleotide (61.7%) and amino acid (53%) identity with the trout IL-1β sequences. The IL-1β message was detected by RT-PCR in head-kidney, blood, spleen, liver, gill and peritoneal exudate of both non-infected and Vibrio anguillarum-challenged fish. More importantly, IL-1β was highly expressed by purified macrophage monolayers and was up-regulated by lipopolysaccharide and lymphocyte-derived macrophage-activating factor stimulation. © 2001 Academic Press.

Original language	English
Pages (from-to)	67-72
Number of pages	5
Journal	Cytokine
Volume	16
Issue number	2
DOIs	https://doi.org/10.1006/cyto.2001.0949
Publication status	Published - 2001

Keywords

Fish
Interleukin-1β
Macrophages
Phylogeny

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10.1006/cyto.2001.0949

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title = "Interleukin-1β isolated from a marine fish reveals up-regulated expression in macrophages following activation with lipopolysaccharide and lymphokines",

abstract = "The gilthead seabream IL-1β gene consists of five exons/four introns. The complete coding sequence contains a 102 bp 5′ untranslated region (UTR), a single open reading frame of 762 bp which translates into a 253 amino acid molecule, and a 407 bp 3′UTR with a polyadenylation signal 14 nucleotides upstream of the poly(A)tail. The seabream sequence has the highest degree of nucleotide (61.7%) and amino acid (53%) identity with the trout IL-1β sequences. The IL-1β message was detected by RT-PCR in head-kidney, blood, spleen, liver, gill and peritoneal exudate of both non-infected and Vibrio anguillarum-challenged fish. More importantly, IL-1β was highly expressed by purified macrophage monolayers and was up-regulated by lipopolysaccharide and lymphocyte-derived macrophage-activating factor stimulation. {\textcopyright} 2001 Academic Press.",

keywords = "Fish, Interleukin-1β, Macrophages, Phylogeny",

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year = "2001",

doi = "10.1006/cyto.2001.0949",

language = "English",

volume = "16",

pages = "67--72",

journal = "Cytokine",

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T1 - Interleukin-1β isolated from a marine fish reveals up-regulated expression in macrophages following activation with lipopolysaccharide and lymphokines

AU - Pelegrín, Pablo

AU - García-Castillo, Jesús

AU - Mulero, Victoriano

AU - Meseguer, José

PY - 2001

Y1 - 2001

N2 - The gilthead seabream IL-1β gene consists of five exons/four introns. The complete coding sequence contains a 102 bp 5′ untranslated region (UTR), a single open reading frame of 762 bp which translates into a 253 amino acid molecule, and a 407 bp 3′UTR with a polyadenylation signal 14 nucleotides upstream of the poly(A)tail. The seabream sequence has the highest degree of nucleotide (61.7%) and amino acid (53%) identity with the trout IL-1β sequences. The IL-1β message was detected by RT-PCR in head-kidney, blood, spleen, liver, gill and peritoneal exudate of both non-infected and Vibrio anguillarum-challenged fish. More importantly, IL-1β was highly expressed by purified macrophage monolayers and was up-regulated by lipopolysaccharide and lymphocyte-derived macrophage-activating factor stimulation. © 2001 Academic Press.

AB - The gilthead seabream IL-1β gene consists of five exons/four introns. The complete coding sequence contains a 102 bp 5′ untranslated region (UTR), a single open reading frame of 762 bp which translates into a 253 amino acid molecule, and a 407 bp 3′UTR with a polyadenylation signal 14 nucleotides upstream of the poly(A)tail. The seabream sequence has the highest degree of nucleotide (61.7%) and amino acid (53%) identity with the trout IL-1β sequences. The IL-1β message was detected by RT-PCR in head-kidney, blood, spleen, liver, gill and peritoneal exudate of both non-infected and Vibrio anguillarum-challenged fish. More importantly, IL-1β was highly expressed by purified macrophage monolayers and was up-regulated by lipopolysaccharide and lymphocyte-derived macrophage-activating factor stimulation. © 2001 Academic Press.

KW - Fish

KW - Interleukin-1β

KW - Macrophages

KW - Phylogeny

U2 - 10.1006/cyto.2001.0949

DO - 10.1006/cyto.2001.0949

M3 - Article

C2 - 11683587

SN - 1043-4666

VL - 16

SP - 67

EP - 72

JO - Cytokine

JF - Cytokine

IS - 2

ER -

Interleukin-1β isolated from a marine fish reveals up-regulated expression in macrophages following activation with lipopolysaccharide and lymphokines

Abstract

Keywords

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