Interleukin 10 genotype as a risk factor for sudden infant death syndrome: Determination of IL-10 genotype from wax-embedded postmortem samples

David Drucker; May Korachi; Vera Pravica; Anthony J. Barson; Ian V. Hutchinson; David B Drucker

doi:10.1016/j.femsim.2004.06.008

Interleukin 10 genotype as a risk factor for sudden infant death syndrome: Determination of IL-10 genotype from wax-embedded postmortem samples

David Drucker, May Korachi, Vera Pravica, Anthony J. Barson, Ian V. Hutchinson, David B Drucker

Research output: Contribution to journal › Article › peer-review

Abstract

In a previous study an association was shown between SIDS and an interleukin-10 (IL-10) genotype. That study was carried out on frozen, unfixed tissue samples, but these are difficult to obtain. Fixed samples used for pathological examination are available. The purpose of this study was to extend the previous work by establishing methods to extract and genotype DNA from fixed, wax-embedded tissues specimens and to use the results to seek confirmation of the association between IL-10 genotype and SIDS in a larger collection of SIDS babies. Using an amplification refractory mutation system-polymerase chain reaction method, a total of 38 infants were genotyped for IL-10 alleles and compared with controls. There was a significant association between the IL-10 -592*A allele and SIDS, consistent with the earlier findings. This study lends support to the hypothesis that IL-10 genotype is related to the susceptibility of babies to SIDS. © 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	125-129
Number of pages	4
Journal	FEMS Immunology and Medical Microbiology
Volume	42
Issue number	1
DOIs	https://doi.org/10.1016/j.femsim.2004.06.008
Publication status	Published - 1 Sept 2004

Keywords

Genotyping
Interleukin 10
SIDS

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.femsim.2004.06.008

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@article{531ea988881544a48e23750d0afc7635,

title = "Interleukin 10 genotype as a risk factor for sudden infant death syndrome: Determination of IL-10 genotype from wax-embedded postmortem samples",

abstract = "In a previous study an association was shown between SIDS and an interleukin-10 (IL-10) genotype. That study was carried out on frozen, unfixed tissue samples, but these are difficult to obtain. Fixed samples used for pathological examination are available. The purpose of this study was to extend the previous work by establishing methods to extract and genotype DNA from fixed, wax-embedded tissues specimens and to use the results to seek confirmation of the association between IL-10 genotype and SIDS in a larger collection of SIDS babies. Using an amplification refractory mutation system-polymerase chain reaction method, a total of 38 infants were genotyped for IL-10 alleles and compared with controls. There was a significant association between the IL-10 -592*A allele and SIDS, consistent with the earlier findings. This study lends support to the hypothesis that IL-10 genotype is related to the susceptibility of babies to SIDS. {\textcopyright} 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.",

keywords = "Genotyping, Interleukin 10, SIDS",

author = "David Drucker and May Korachi and Vera Pravica and Barson, {Anthony J.} and Hutchinson, {Ian V.} and {B Drucker}, David",

year = "2004",

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doi = "10.1016/j.femsim.2004.06.008",

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T1 - Interleukin 10 genotype as a risk factor for sudden infant death syndrome: Determination of IL-10 genotype from wax-embedded postmortem samples

AU - Drucker, David

AU - Korachi, May

AU - Pravica, Vera

AU - Barson, Anthony J.

AU - Hutchinson, Ian V.

AU - B Drucker, David

PY - 2004/9/1

Y1 - 2004/9/1

N2 - In a previous study an association was shown between SIDS and an interleukin-10 (IL-10) genotype. That study was carried out on frozen, unfixed tissue samples, but these are difficult to obtain. Fixed samples used for pathological examination are available. The purpose of this study was to extend the previous work by establishing methods to extract and genotype DNA from fixed, wax-embedded tissues specimens and to use the results to seek confirmation of the association between IL-10 genotype and SIDS in a larger collection of SIDS babies. Using an amplification refractory mutation system-polymerase chain reaction method, a total of 38 infants were genotyped for IL-10 alleles and compared with controls. There was a significant association between the IL-10 -592*A allele and SIDS, consistent with the earlier findings. This study lends support to the hypothesis that IL-10 genotype is related to the susceptibility of babies to SIDS. © 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

AB - In a previous study an association was shown between SIDS and an interleukin-10 (IL-10) genotype. That study was carried out on frozen, unfixed tissue samples, but these are difficult to obtain. Fixed samples used for pathological examination are available. The purpose of this study was to extend the previous work by establishing methods to extract and genotype DNA from fixed, wax-embedded tissues specimens and to use the results to seek confirmation of the association between IL-10 genotype and SIDS in a larger collection of SIDS babies. Using an amplification refractory mutation system-polymerase chain reaction method, a total of 38 infants were genotyped for IL-10 alleles and compared with controls. There was a significant association between the IL-10 -592*A allele and SIDS, consistent with the earlier findings. This study lends support to the hypothesis that IL-10 genotype is related to the susceptibility of babies to SIDS. © 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

KW - Genotyping

KW - Interleukin 10

KW - SIDS

U2 - 10.1016/j.femsim.2004.06.008

DO - 10.1016/j.femsim.2004.06.008

M3 - Article

SN - 0928-8244

VL - 42

SP - 125

EP - 129

JO - FEMS Immunology and Medical Microbiology

JF - FEMS Immunology and Medical Microbiology

IS - 1

ER -

Interleukin 10 genotype as a risk factor for sudden infant death syndrome: Determination of IL-10 genotype from wax-embedded postmortem samples

Abstract

Keywords

UN SDGs

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