Interleukin-6, IL-6 receptor, and IL-6 nuclear factor gene expression in Paget's disease.

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    Abstract

    The cytokine interleukin-6 (IL-6) is considered an important regulator of bone cell function and may play a central role in bone disease states characterized by increased bone remodeling, such as Paget's disease. Indeed, recent in vitro data suggest that IL-6 may be an autocrine/paracrine factor for pagetic osteoclasts. However, its expression and role in vivo are not known. Using in situ hybridization we investigated the spatial localization of expression of IL-6, IL-6 receptor (IL-6R), and the transcription factor (NF-IL-6) in pagetic bone. Our results show that osteoblasts in the normal remodeling bone of osteoarthritis (controls) and in Paget's disease express IL-6, IL-6R, and NF-IL-6 genes with higher levels of IL-6 and IL-6R mRNA in pagetic bone. Osteoclasts in both osteoarthritic and pagetic bone express IL-6R mRNA and NF-IL-6, but only pagetic osteoclasts expressed IL-6, suggesting that in Paget's disease IL-6 can act as an autocrine factor on osteoclasts. These results provide evidence for a major role of the IL-6 regulatory pathway in the phenotype of the pagetic osteoclasts and lead us to suggest a model linking possible paramyxovirus infection and IL-6 regulation in the pagetic osteoclast.
    Original languageEnglish
    JournalJ Bone Miner Res
    Volume9
    Issue number1
    DOIs
    Publication statusPublished - Jan 1994

    Keywords

    • Bone Remodeling
    • metabolism: Bone and Bones
    • CCAAT-Enhancer-Binding Proteins
    • biosynthesis: DNA-Binding Proteins
    • Gene Expression
    • Humans
    • In Situ Hybridization
    • biosynthesis: Interleukin-6
    • biosynthesis: Nuclear Proteins
    • genetics: Osteitis Deformans
    • metabolism: Osteoarthritis
    • metabolism: Osteoblasts
    • metabolism: Osteoclasts
    • Phenotype
    • genetics: RNA, Messenger
    • biosynthesis: Receptors, Interleukin
    • Receptors, Interleukin-6
    • Research Support, Non-U.S. Gov't
    • physiology: Respirovirus

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