Internal electron transfer in multi-site redox enzymes is accessed by laser excitation of thiouredopyrene-3,6,8-trisulfonate (TUPS)

Derren J. Heyes; Anne Marie Quinn; Paul M. Cullis; Michael Lee; Andrew W. Munro; Nigel S. Scrutton

doi:10.1039/b820386e

Internal electron transfer in multi-site redox enzymes is accessed by laser excitation of thiouredopyrene-3,6,8-trisulfonate (TUPS)

Derren J. Heyes
, Anne Marie Quinn
, Paul M. Cullis
, Michael Lee
, Andrew W. Munro
, Nigel S. Scrutton

Research output: Contribution to journal › Article › peer-review

Abstract

We demonstrate that thiouredopyrene-3,6,8-trisulfonate (TUPS), a photoactivatable reagent, can rapidly inject electrons into complex redox enzymes, enabling studies of the kinetics of internal electron that are not accessible using conventional rapid mixing, stopped-flow methods. © The Royal Society of Chemistry 2009.

Original language	English
Pages (from-to)	1124-1126
Number of pages	2
Journal	Chemical Communications
Issue number	9
DOIs	https://doi.org/10.1039/b820386e
Publication status	Published - 2009

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@article{2437164e2384441b943644e8627b6cb3,

title = "Internal electron transfer in multi-site redox enzymes is accessed by laser excitation of thiouredopyrene-3,6,8-trisulfonate (TUPS)",

abstract = "We demonstrate that thiouredopyrene-3,6,8-trisulfonate (TUPS), a photoactivatable reagent, can rapidly inject electrons into complex redox enzymes, enabling studies of the kinetics of internal electron that are not accessible using conventional rapid mixing, stopped-flow methods. {\textcopyright} The Royal Society of Chemistry 2009.",

author = "Heyes, \{Derren J.\} and Quinn, \{Anne Marie\} and Cullis, \{Paul M.\} and Michael Lee and Munro, \{Andrew W.\} and Scrutton, \{Nigel S.\}",

year = "2009",

doi = "10.1039/b820386e",

language = "English",

pages = "1124--1126",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "9",

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TY - JOUR

T1 - Internal electron transfer in multi-site redox enzymes is accessed by laser excitation of thiouredopyrene-3,6,8-trisulfonate (TUPS)

AU - Heyes, Derren J.

AU - Quinn, Anne Marie

AU - Cullis, Paul M.

AU - Lee, Michael

AU - Munro, Andrew W.

AU - Scrutton, Nigel S.

PY - 2009

Y1 - 2009

N2 - We demonstrate that thiouredopyrene-3,6,8-trisulfonate (TUPS), a photoactivatable reagent, can rapidly inject electrons into complex redox enzymes, enabling studies of the kinetics of internal electron that are not accessible using conventional rapid mixing, stopped-flow methods. © The Royal Society of Chemistry 2009.

AB - We demonstrate that thiouredopyrene-3,6,8-trisulfonate (TUPS), a photoactivatable reagent, can rapidly inject electrons into complex redox enzymes, enabling studies of the kinetics of internal electron that are not accessible using conventional rapid mixing, stopped-flow methods. © The Royal Society of Chemistry 2009.

U2 - 10.1039/b820386e

DO - 10.1039/b820386e

M3 - Article

C2 - 19225657

SN - 1359-7345

SP - 1124

EP - 1126

JO - Chemical Communications

JF - Chemical Communications

IS - 9

ER -

Internal electron transfer in multi-site redox enzymes is accessed by laser excitation of thiouredopyrene-3,6,8-trisulfonate (TUPS)

Abstract

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