Introduction of new genetic material into pluripotent haematopoietic stem cells of the mouse.

D A Williams, I R Lemischka, D G Nathan, R C Mulligan

    Research output: Contribution to journalArticlepeer-review

    Abstract

    An infectious retrovirus vector has been used to transfer a bacterial gene encoding resistance to the neomycin analogue G418 into pluripotent haematopoietic stem cells present in explanted murine bone marrow tissue. Subsequent transplantation of the cells into lethally irradiated mice results in engraftment of the animals with donor haematopoietic tissue containing the bacterial gene. This approach affords an efficient and rapid means of re-introducing genetically modified tissue into intact organisms and provides a system whereby the expression and regulation of cloned genes can be followed within the context of a well characterized developmental programme.
    Original languageEnglish
    Pages (from-to)476-480
    JournalNature
    Volume310
    Issue number5977
    DOIs
    Publication statusPublished - Aug 1984

    Fingerprint

    Dive into the research topics of 'Introduction of new genetic material into pluripotent haematopoietic stem cells of the mouse.'. Together they form a unique fingerprint.

    Cite this