@inbook{3b064547d7024f22a6a4255bdd2d6ff7,
title = "Investigating IL-1β Secretion Using Real-Time Single-Cell Imaging.",
abstract = "The pro-inflammatory cytokine interleukin (IL)-1β is an important mediator of the inflammatory response. In order to perform its role in the inflammatory cascade, IL-1β must be secreted from the cell, yet it lacks a signal peptide that is required for conventional secretion, and the exact mechanism of release remains undefined. Conventional biochemical methods have limited the investigation into the processes involved in IL-1β secretion to population dynamics, yet heterogeneity between cells has been observed at a single-cell level. Here, greater sensitivity is achieved with the use of a newly developed vector that codes for a fluorescently labelled version of IL-1β. Combining this with real-time single-cell confocal microscopy using the methods described here, we have developed an effective protocol for investigating the mechanisms of IL-1β secretion and the testing of the hypothesis that IL-1β secretion requires membrane permeabilisation. ",
author = "Catherine Diamond and James Bagnall and David Spiller and Michael White and Alessandra Mortellaro and Pawel Paszek and David Brough",
year = "2016",
doi = "10.1007/978-1-4939-3566-6_4",
language = "English",
isbn = "978-1-4939-3564-2",
series = "Methods in Molecular Biology",
publisher = "Springer Nature",
pages = "75--88",
editor = "{Di Virgilio}, Francesco and Pelegr{\'i}n Pablo",
booktitle = "NLR proteins",
address = "United States",
}