Investigating the interactions of hyaluronan derivatives with biomolecules. The use of diffusional NMR techniques

Anna Taglienti, Francesco Cellesi, Vittorio Crescenzi, Paolo Sequi, Massimiliano Valentini, Nicola Tirelli

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The interactions between a biomaterial and biomolecules present in body fluids often determine the fate of the biomaterial. This paper presents a study on hyaluronan (HA)-containing materials (in soluble or colloidal form) that focuses on their interactions with lipids and proteins and for the first time uses PFG NMR as an analytical technique for probing these events. The interactions of HA-based polymers with phospholipids (DPPC and DPPG liposomes) are shown to depend both on charge and hydrophobicity factors. Despite the difference in behavior between albumin (substantially non-adhesive) and fibrinogen (adhesive), the interactions of the polymers with proteins do not seem to be based on hydrophobic effects but on surface polar interactions. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.
    Original languageEnglish
    Pages (from-to)611-622
    Number of pages11
    JournalMacromolecular Bioscience
    Volume6
    Issue number8
    DOIs
    Publication statusPublished - 7 Aug 2006

    Keywords

    • "Stealth" character
    • Colloidal carriers
    • Hyaluronan
    • PFG NMR
    • Protein adsorption

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