TY - JOUR
T1 - Investigation of K(ATP) channel endocytosis and cell surface density by Biotinylation and Western blotting.
AU - Smith, Andrew J
AU - Sivaprasadarao, Asipu
PY - 2008
Y1 - 2008
N2 - Macroscopic ion channel currents (I) are a product of the channel open probability (P(o)), the single channel current (i) and the number of channels present on the cell surface (N) at any given time (I = P(o)iN). Endocytosis has been shown to be one of the key determinants of cell surface channel density and defects of this process have been linked to diseases relating to ion channel dysfunction. Biotinylation allows the selective labelling and isolation of surface exposed proteins which can then be identified by Western blotting.
AB - Macroscopic ion channel currents (I) are a product of the channel open probability (P(o)), the single channel current (i) and the number of channels present on the cell surface (N) at any given time (I = P(o)iN). Endocytosis has been shown to be one of the key determinants of cell surface channel density and defects of this process have been linked to diseases relating to ion channel dysfunction. Biotinylation allows the selective labelling and isolation of surface exposed proteins which can then be identified by Western blotting.
UR - https://www.scopus.com/pages/publications/60349131987
U2 - 10.1007/978-1-59745-526-8_6
DO - 10.1007/978-1-59745-526-8_6
M3 - Article
C2 - 18998085
SN - 1064-3745
VL - 491
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -