Investigation on the properties of poly--caprolactone solvent casted films after the surface modification as a potential material for nerve regeneration.

Alba Carla De Luca; G. Terenghi; S. Downes

Investigation on the properties of poly--caprolactone solvent casted films after the surface modification as a potential material for nerve regeneration.

Alba Carla De Luca, G. Terenghi, S. Downes

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Introduction: Poly--caprolactone (PCL) is a biocompatible and biodegradable polymer suitable for tissue engineering. Solvent casted films are characterised by a particular micropitted surface. Previous works demonstrated that neural cells are able to adhere and proliferate on these scaffolds. However, surface modification is necessary in order to improve the material biocompatibility. Particularly, hydrolysis and the presence of functional groups have been found to influence the cell-material interaction.Methods: A solution of PCL/dichloromethane 3% wt/v was casted on glass coverslips and the solvent was left to evaporate. Films were treated with three different solutions: sodium hydroxide (NaOH) 10M, potassium hydroxide (KOH) 10M and hexamethylendiamine (HMD) in isopropanol (IPA) 10% wt/v. Mechanical tensile tests were carried out (n=5) to characterise the degradation of the material and a NG108-15 cells proliferation assay using Alamar Blue was carried out for 6 days (n=3) to investigate the films biocompatibility.Results: A remarkable decrease (p

Original language	English
Title of host publication	host publication
Place of Publication	Spain
Publisher	Histology and Histopathology
Publication status	Published - Jun 2011
Event	TERMIS 2011 - Granada, Spain Duration: 7 Jun 2011 → 10 Jun 2011

Conference

Conference	TERMIS 2011
City	Granada, Spain
Period	7/06/11 → 10/06/11

Keywords

biocompatibility, surface modification, cells response

Cite this

@inproceedings{d4ef18c17f13436ba908e13ef58b4596,

title = "Investigation on the properties of poly--caprolactone solvent casted films after the surface modification as a potential material for nerve regeneration.",

abstract = "Introduction: Poly--caprolactone (PCL) is a biocompatible and biodegradable polymer suitable for tissue engineering. Solvent casted films are characterised by a particular micropitted surface. Previous works demonstrated that neural cells are able to adhere and proliferate on these scaffolds. However, surface modification is necessary in order to improve the material biocompatibility. Particularly, hydrolysis and the presence of functional groups have been found to influence the cell-material interaction.Methods: A solution of PCL/dichloromethane 3% wt/v was casted on glass coverslips and the solvent was left to evaporate. Films were treated with three different solutions: sodium hydroxide (NaOH) 10M, potassium hydroxide (KOH) 10M and hexamethylendiamine (HMD) in isopropanol (IPA) 10% wt/v. Mechanical tensile tests were carried out (n=5) to characterise the degradation of the material and a NG108-15 cells proliferation assay using Alamar Blue was carried out for 6 days (n=3) to investigate the films biocompatibility.Results: A remarkable decrease (p",

keywords = "biocompatibility, surface modification, cells response",

author = "{De Luca}, {Alba Carla} and G. Terenghi and S. Downes",

year = "2011",

month = jun,

language = "English",

booktitle = "host publication",

publisher = "Histology and Histopathology",

address = "Spain",

note = "TERMIS 2011 ; Conference date: 07-06-2011 Through 10-06-2011",

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Investigation on the properties of poly--caprolactone solvent casted films after the surface modification as a potential material for nerve regeneration. / De Luca, Alba Carla; Terenghi, G.; Downes, S.
host publication. Spain: Histology and Histopathology, 2011.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Investigation on the properties of poly--caprolactone solvent casted films after the surface modification as a potential material for nerve regeneration.

AU - De Luca, Alba Carla

AU - Terenghi, G.

AU - Downes, S.

PY - 2011/6

Y1 - 2011/6

N2 - Introduction: Poly--caprolactone (PCL) is a biocompatible and biodegradable polymer suitable for tissue engineering. Solvent casted films are characterised by a particular micropitted surface. Previous works demonstrated that neural cells are able to adhere and proliferate on these scaffolds. However, surface modification is necessary in order to improve the material biocompatibility. Particularly, hydrolysis and the presence of functional groups have been found to influence the cell-material interaction.Methods: A solution of PCL/dichloromethane 3% wt/v was casted on glass coverslips and the solvent was left to evaporate. Films were treated with three different solutions: sodium hydroxide (NaOH) 10M, potassium hydroxide (KOH) 10M and hexamethylendiamine (HMD) in isopropanol (IPA) 10% wt/v. Mechanical tensile tests were carried out (n=5) to characterise the degradation of the material and a NG108-15 cells proliferation assay using Alamar Blue was carried out for 6 days (n=3) to investigate the films biocompatibility.Results: A remarkable decrease (p

AB - Introduction: Poly--caprolactone (PCL) is a biocompatible and biodegradable polymer suitable for tissue engineering. Solvent casted films are characterised by a particular micropitted surface. Previous works demonstrated that neural cells are able to adhere and proliferate on these scaffolds. However, surface modification is necessary in order to improve the material biocompatibility. Particularly, hydrolysis and the presence of functional groups have been found to influence the cell-material interaction.Methods: A solution of PCL/dichloromethane 3% wt/v was casted on glass coverslips and the solvent was left to evaporate. Films were treated with three different solutions: sodium hydroxide (NaOH) 10M, potassium hydroxide (KOH) 10M and hexamethylendiamine (HMD) in isopropanol (IPA) 10% wt/v. Mechanical tensile tests were carried out (n=5) to characterise the degradation of the material and a NG108-15 cells proliferation assay using Alamar Blue was carried out for 6 days (n=3) to investigate the films biocompatibility.Results: A remarkable decrease (p

KW - biocompatibility, surface modification, cells response

M3 - Conference contribution

BT - host publication

PB - Histology and Histopathology

CY - Spain

T2 - TERMIS 2011

Y2 - 7 June 2011 through 10 June 2011

ER -

Investigation on the properties of poly--caprolactone solvent casted films after the surface modification as a potential material for nerve regeneration.

Abstract

Conference

Keywords

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