In vitro differentiation of human embryonic stem cells to hemogenic endothelium and blood progenitors via embryoid body formation

Eva Garcia-Alegria; Bethany Potts; Sara Menegatti; Valerie Kouskoff

doi:10.1016/j.xpro.2021.100367

In vitro differentiation of human embryonic stem cells to hemogenic endothelium and blood progenitors via embryoid body formation

Eva Garcia-Alegria, Bethany Potts, Sara Menegatti, Valerie Kouskoff

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Abstract

Little is known about the emergence of blood progenitors during human embryogenesis due to ethical reasons and restricted embryo access. The use of human embryonic stem cells (hESCs) as a model system offers unique opportunities to dissect human blood cell formation. Here, we describe a protocol allowing the differentiation of hESCs via embryoid bodies toward hemogenic endothelium and its subsequent differentiation to blood progenitors. This protocol relies on the formation of embryoid bodies, which is tricky if not carefully performed.

Original language	English
Article number	100367
Journal	STAR protocols
Volume	2
Issue number	1
Early online date	2 Mar 2021
DOIs	https://doi.org/10.1016/j.xpro.2021.100367
Publication status	Published - 19 Mar 2021

Keywords

Cell culture
Cell differentiation
Cell isolation
Flow cytometry/mass cytometry
Stem cells

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10.1016/j.xpro.2021.100367Licence: CC BY-NC-ND

1-s2.0-S2666166721000745-mainFinal published version, 5.54 MBLicence: CC BY-NC-ND

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abstract = "Little is known about the emergence of blood progenitors during human embryogenesis due to ethical reasons and restricted embryo access. The use of human embryonic stem cells (hESCs) as a model system offers unique opportunities to dissect human blood cell formation. Here, we describe a protocol allowing the differentiation of hESCs via embryoid bodies toward hemogenic endothelium and its subsequent differentiation to blood progenitors. This protocol relies on the formation of embryoid bodies, which is tricky if not carefully performed.",

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AU - Kouskoff, Valerie

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KW - Cell differentiation

KW - Cell isolation

KW - Flow cytometry/mass cytometry

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In vitro differentiation of human embryonic stem cells to hemogenic endothelium and blood progenitors via embryoid body formation

Abstract

Keywords

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