Isolation and characterization of melanopsin (Opn4) from the Australian marsupial Sminthopsis crassicaudata (fat-tailed dunnart)

Susana S. Pires, Julia Shand, James Bellingham, Catherine Arrese, Michael Turton, Stuart Peirson, Russell G. Foster, Stephanie Halford

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Melanopsin confers photosensitivity to a subset of retinal ganglion cells and is responsible for many non-image-forming tasks, like the detection of light for circadian entrainment. Recently, two melanopsin genes, Opn4m and Opn4x, were described in non-mammalian vertebrates. However, only one form, Opn4m, has been described in the mammals, although studies to date have been limited to the placentals and have not included the marsupials. We report here the isolation and characterization of an Opn4 gene from an Australian marsupial, the fattailed dunnart (Sminthopsis crassicaudata), and present evidence which suggests that the Opn4x gene was lost before the placental/marsupial split. In situ hybridization shows that the expression of Opn4 in the dunnart eye is restricted to a subset of ganglion cells, a pattern previously reported for rodents and primates. These Opn4-positive cells are randomly distributed across the dunnart retina. We also undertook a comparative analysis with the South American marsupial, the grey short-tailed opossum (Monodelphis domestica), and two placental mammals, mouse and human. This approach reveals that the two marsupials show a higher sequence identity than that seen between rodents and primates, despite separating at approximately the same point in time, some 65-85 Myr ago. © 2007 The Royal Society.
    Original languageEnglish
    Pages (from-to)2791-2799
    Number of pages8
    JournalProceedings of the Royal Society B: Biological Sciences
    Volume274
    Issue number1627
    DOIs
    Publication statusPublished - 22 Nov 2007

    Keywords

    • Circadian entrainment
    • Ganglion cell
    • Marsupial
    • Melanopsin
    • Phylogeny
    • Retina

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