Isolation and molecular characterization of polyvinyl chloride (PVC) plastic degrading fungal isolates

Muhammad Ishtiaq Ali; Safia Ahmed; Geoff Robson; Imran Javed; Naeem Ali; Naima Atiq; Abdul Hameed

doi:10.1002/jobm.201200496

Isolation and molecular characterization of polyvinyl chloride (PVC) plastic degrading fungal isolates

Muhammad Ishtiaq Ali, Safia Ahmed, Geoff Robson, Imran Javed, Naeem Ali, Naima Atiq, Abdul Hameed

Research output: Contribution to journal › Article › peer-review

Abstract

The recalcitrant nature of polyvinyl chloride creates serious environmental concerns during manufacturing and waste disposal. The present study was aimed to isolate and screen different soil fungi having potential to biodegrade PVC films. After 10 months of soil burial experiment, it was observed that a number of fungal strains were flourishing on PVC films. On morphological as well as on 18rRNA gene sequence and phylogenetic basis they were identified as Phanerochaete chrysosporium PV1, Lentinus tigrinus PV2, Aspergillus niger PV3, and Aspergillus sydowii PV4. The biodegradation ability of these fungal isolates was further checked in shake flask experiments by taking thin films of PVC (C source) in mineral salt medium. A significant change in color and surface deterioration of PVC films was confirmed through visual observation and Scanning electron microscopy. During shake flask experiments, P. chrysosporium PV1 produced maximum biomass of about 2.57mgml-1 followed by A. niger PV3. P. chrysosporium PV1 showed significant reduction (178,292Da-1) in Molecular weight of the PVC film than control (200,000Da-1) by gel permeation chromatography. Furthermore more Fourier transform infrared spectroscopy and nuclear magnetic resonance also revealed structural changes in the PVC. It was concluded that isolated fungal strains have significant potential for biodegradation of PVC plastics. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Original language	English
Pages (from-to)	18-27
Number of pages	9
Journal	Journal of Basic Microbiology
Volume	54
Issue number	1
DOIs	https://doi.org/10.1002/jobm.201200496
Publication status	Published - Jan 2014

Keywords

Biodegradation
Fungi
GPC
Polyvinyl chloride
SEM

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/jobm.201200496

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title = "Isolation and molecular characterization of polyvinyl chloride (PVC) plastic degrading fungal isolates",

abstract = "The recalcitrant nature of polyvinyl chloride creates serious environmental concerns during manufacturing and waste disposal. The present study was aimed to isolate and screen different soil fungi having potential to biodegrade PVC films. After 10 months of soil burial experiment, it was observed that a number of fungal strains were flourishing on PVC films. On morphological as well as on 18rRNA gene sequence and phylogenetic basis they were identified as Phanerochaete chrysosporium PV1, Lentinus tigrinus PV2, Aspergillus niger PV3, and Aspergillus sydowii PV4. The biodegradation ability of these fungal isolates was further checked in shake flask experiments by taking thin films of PVC (C source) in mineral salt medium. A significant change in color and surface deterioration of PVC films was confirmed through visual observation and Scanning electron microscopy. During shake flask experiments, P. chrysosporium PV1 produced maximum biomass of about 2.57mgml-1 followed by A. niger PV3. P. chrysosporium PV1 showed significant reduction (178,292Da-1) in Molecular weight of the PVC film than control (200,000Da-1) by gel permeation chromatography. Furthermore more Fourier transform infrared spectroscopy and nuclear magnetic resonance also revealed structural changes in the PVC. It was concluded that isolated fungal strains have significant potential for biodegradation of PVC plastics. {\textcopyright} 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",

keywords = "Biodegradation, Fungi, GPC, Polyvinyl chloride, SEM",

author = "Ali, {Muhammad Ishtiaq} and Safia Ahmed and Geoff Robson and Imran Javed and Naeem Ali and Naima Atiq and Abdul Hameed",

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AU - Ali, Muhammad Ishtiaq

AU - Ahmed, Safia

AU - Robson, Geoff

AU - Javed, Imran

AU - Ali, Naeem

AU - Atiq, Naima

AU - Hameed, Abdul

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N2 - The recalcitrant nature of polyvinyl chloride creates serious environmental concerns during manufacturing and waste disposal. The present study was aimed to isolate and screen different soil fungi having potential to biodegrade PVC films. After 10 months of soil burial experiment, it was observed that a number of fungal strains were flourishing on PVC films. On morphological as well as on 18rRNA gene sequence and phylogenetic basis they were identified as Phanerochaete chrysosporium PV1, Lentinus tigrinus PV2, Aspergillus niger PV3, and Aspergillus sydowii PV4. The biodegradation ability of these fungal isolates was further checked in shake flask experiments by taking thin films of PVC (C source) in mineral salt medium. A significant change in color and surface deterioration of PVC films was confirmed through visual observation and Scanning electron microscopy. During shake flask experiments, P. chrysosporium PV1 produced maximum biomass of about 2.57mgml-1 followed by A. niger PV3. P. chrysosporium PV1 showed significant reduction (178,292Da-1) in Molecular weight of the PVC film than control (200,000Da-1) by gel permeation chromatography. Furthermore more Fourier transform infrared spectroscopy and nuclear magnetic resonance also revealed structural changes in the PVC. It was concluded that isolated fungal strains have significant potential for biodegradation of PVC plastics. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

AB - The recalcitrant nature of polyvinyl chloride creates serious environmental concerns during manufacturing and waste disposal. The present study was aimed to isolate and screen different soil fungi having potential to biodegrade PVC films. After 10 months of soil burial experiment, it was observed that a number of fungal strains were flourishing on PVC films. On morphological as well as on 18rRNA gene sequence and phylogenetic basis they were identified as Phanerochaete chrysosporium PV1, Lentinus tigrinus PV2, Aspergillus niger PV3, and Aspergillus sydowii PV4. The biodegradation ability of these fungal isolates was further checked in shake flask experiments by taking thin films of PVC (C source) in mineral salt medium. A significant change in color and surface deterioration of PVC films was confirmed through visual observation and Scanning electron microscopy. During shake flask experiments, P. chrysosporium PV1 produced maximum biomass of about 2.57mgml-1 followed by A. niger PV3. P. chrysosporium PV1 showed significant reduction (178,292Da-1) in Molecular weight of the PVC film than control (200,000Da-1) by gel permeation chromatography. Furthermore more Fourier transform infrared spectroscopy and nuclear magnetic resonance also revealed structural changes in the PVC. It was concluded that isolated fungal strains have significant potential for biodegradation of PVC plastics. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

KW - Biodegradation

KW - Fungi

KW - GPC

KW - Polyvinyl chloride

KW - SEM

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DO - 10.1002/jobm.201200496

M3 - Article

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JO - Journal of Basic Microbiology

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IS - 1

ER -

Isolation and molecular characterization of polyvinyl chloride (PVC) plastic degrading fungal isolates

Abstract

Keywords

UN SDGs

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