Isolation of a Chlamydomonas reinhardtii telomere by functional complementation in yeast

T. Hails, O. Huttner, A. Day

    Research output: Contribution to journalArticlepeer-review

    Abstract

    We attempted to determine whether Chlamydomonas reinhardtii telomeres, which do not form G-quartet structures readily in vitro, are able to nucleate telomere addition in Saccharomyces cerevisiae. Restricted C. reinhardtii genomic DNA was ligated to a linear S. cerevisiae vector lacking a telomere. A C. reinhardtii telomere ligated to this unprotected end allowed vector replication as a linear DNA molecule in S. cerevisiae, DNA sequencing revealed common [T4AG3](n) and variant T6AG3 and T5AG3 C. reinhardtii telomere repeats capped by S. cerevisiae telomere repeat units. The recognition of a C. reinhardtii telomere by the telomere maintenance machinery of S. cerevisiae is consistent with a common theme for telomere structure in organisms with divergent telomere repeats.
    Original languageEnglish
    Pages (from-to)437-440
    Number of pages3
    JournalCurrent Genetics
    Volume28
    Issue number5
    DOIs
    Publication statusPublished - 1995

    Keywords

    • C. reinhardtii
    • Functional complementation
    • S. cerevisiae
    • Telomere

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