­­­Kidney organoids recapitulate human basement membrane assembly in health and disease

Mychel Rpt Morais, Pinyuan Tian, Craig Lawless, Syed Murtuza-baker, Louise Hopkinson, Steven Woods, Aleksandr Mironov, David A Long, Daniel P Gale, Telma Mt Zorn, Susan J Kimber, Roy Zent, Rachel Lennon

Research output: Contribution to journalArticlepeer-review


Basement membranes (BMs) are complex macromolecular networks underlying all continuous layers of cells. Essential components include collagen IV and laminins, which are affected by human genetic variants leading to a range of debilitating conditions including kidney, muscle, and cerebrovascular phenotypes. We investigated the dynamics of BM assembly in human pluripotent stem cell-derived kidney organoids. We resolved their global BM composition and discovered a conserved temporal sequence in BM assembly that paralleled mammalian fetal kidneys. We identified the emergence of key BM isoforms, which were altered by a pathogenic variant in COL4A5. Integrating organoid, fetal and adult kidney proteomes we found dynamic regulation of BM composition through development to adulthood, and with single-cell transcriptomic analysis we mapped the cellular origins of BM components. Overall, we define the complex and dynamic nature of kidney BM assembly and provide a platform for understanding its wider relevance in human development and disease.
Original languageEnglish
Early online date25 Jan 2022
Publication statusPublished - 25 Jan 2022


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