Large scale active-learning-guided exploration to maximize cell-free production

Olivier Borkowski, Mathilde Koch, Agnès Zettor, Amir Pandi, Angelo Cardoso Batista, Paul Soudier, Jean-Loup Faulon

Research output: Contribution to journalArticle


Lysate-based cell-free systems have become a major platform to study gene expression but batch-to-batch variation makes protein production difficult to predict. Here we describe an active learning approach to explore a combinatorial space of ~4,000,000 cell-free compositions, maximizing protein production and identifying critical parameters involved in cell-free productivity. We also provide a one-step-method to achieve high quality predictions for protein production using minimal experimental effort regardless of the lysate quality.
Original languageEnglish
Pages (from-to)751669
Publication statusPublished - 2019


Dive into the research topics of 'Large scale active-learning-guided exploration to maximize cell-free production'. Together they form a unique fingerprint.

Cite this