LC-ICP-MS analysis of inositol phosphate isomers in soil offers improved sensitivity and fine-scale mapping of inositol phosphate distribution

Joseph J. Carroll; Colleen Sprigg; Graham Chilvers; Ignacio Delso; Megan Barker; Filipa Cox; David Johnson; Charles A. Brearley

doi:10.1111/2041-210X.14292

LC-ICP-MS analysis of inositol phosphate isomers in soil offers improved sensitivity and fine-scale mapping of inositol phosphate distribution

Joseph J. Carroll, Colleen Sprigg, Graham Chilvers, Ignacio Delso, Megan Barker, Filipa Cox, David Johnson, Charles A. Brearley^*

^*Corresponding author for this work

University of East Anglia

Research output: Contribution to journal › Article › peer-review

Abstract

Organic forms of phosphorus (P) prevail in soils and their quantification is vital to better understand global biogeochemical cycles. P speciation in soil is commonly assessed by ³¹P NMR spectroscopy of sodium hydroxide-EDTA (NaOH-EDTA) extracts. A liquid chromatography-inductively coupled plasma-mass spectrometry (LC-ICP-MS) method that employs NaOH-EDTA is described. Comparison with 31P NMR shows that LC-ICP-MS is up to three orders of magnitude more sensitive. It allows measurement in samples as small as 1 mg. We reveal variation of inositol phosphate distribution in Swedish boreal forest soil and identify myo- and scyllo-inositol hexakisphosphates and other isomers including scyllo-inositol pentakisphosphate. Speciation of the major inositol phosphates was not altered by long-term nitrogen fertilization.

Original language	English
Pages (from-to)	530-543
Number of pages	14
Journal	Methods in Ecology and Evolution
Volume	15
Issue number	3
Early online date	4 Feb 2024
DOIs	https://doi.org/10.1111/2041-210X.14292
Publication status	Published - 1 Mar 2024

Keywords

inositol phosphate isomers
LC-ICP-MS
nitrogen fertilization
phosphorus speciation
phytate
soil 31P NMR

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10.1111/2041-210X.14292Licence: CC BY

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title = "LC-ICP-MS analysis of inositol phosphate isomers in soil offers improved sensitivity and fine-scale mapping of inositol phosphate distribution",

abstract = "Organic forms of phosphorus (P) prevail in soils and their quantification is vital to better understand global biogeochemical cycles. P speciation in soil is commonly assessed by 31P NMR spectroscopy of sodium hydroxide-EDTA (NaOH-EDTA) extracts. A liquid chromatography-inductively coupled plasma-mass spectrometry (LC-ICP-MS) method that employs NaOH-EDTA is described. Comparison with 31P NMR shows that LC-ICP-MS is up to three orders of magnitude more sensitive. It allows measurement in samples as small as 1 mg. We reveal variation of inositol phosphate distribution in Swedish boreal forest soil and identify myo- and scyllo-inositol hexakisphosphates and other isomers including scyllo-inositol pentakisphosphate. Speciation of the major inositol phosphates was not altered by long-term nitrogen fertilization.",

keywords = "inositol phosphate isomers, LC-ICP-MS, nitrogen fertilization, phosphorus speciation, phytate, soil 31P NMR",

author = "Carroll, {Joseph J.} and Colleen Sprigg and Graham Chilvers and Ignacio Delso and Megan Barker and Filipa Cox and David Johnson and Brearley, {Charles A.}",

note = "Publisher Copyright: {\textcopyright} 2024 The Authors. Methods in Ecology and Evolution published by John Wiley & Sons Ltd on behalf of British Ecological Society.",

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doi = "10.1111/2041-210X.14292",

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T1 - LC-ICP-MS analysis of inositol phosphate isomers in soil offers improved sensitivity and fine-scale mapping of inositol phosphate distribution

AU - Carroll, Joseph J.

AU - Sprigg, Colleen

AU - Chilvers, Graham

AU - Delso, Ignacio

AU - Barker, Megan

AU - Cox, Filipa

AU - Johnson, David

AU - Brearley, Charles A.

PY - 2024/3/1

Y1 - 2024/3/1

N2 - Organic forms of phosphorus (P) prevail in soils and their quantification is vital to better understand global biogeochemical cycles. P speciation in soil is commonly assessed by 31P NMR spectroscopy of sodium hydroxide-EDTA (NaOH-EDTA) extracts. A liquid chromatography-inductively coupled plasma-mass spectrometry (LC-ICP-MS) method that employs NaOH-EDTA is described. Comparison with 31P NMR shows that LC-ICP-MS is up to three orders of magnitude more sensitive. It allows measurement in samples as small as 1 mg. We reveal variation of inositol phosphate distribution in Swedish boreal forest soil and identify myo- and scyllo-inositol hexakisphosphates and other isomers including scyllo-inositol pentakisphosphate. Speciation of the major inositol phosphates was not altered by long-term nitrogen fertilization.

AB - Organic forms of phosphorus (P) prevail in soils and their quantification is vital to better understand global biogeochemical cycles. P speciation in soil is commonly assessed by 31P NMR spectroscopy of sodium hydroxide-EDTA (NaOH-EDTA) extracts. A liquid chromatography-inductively coupled plasma-mass spectrometry (LC-ICP-MS) method that employs NaOH-EDTA is described. Comparison with 31P NMR shows that LC-ICP-MS is up to three orders of magnitude more sensitive. It allows measurement in samples as small as 1 mg. We reveal variation of inositol phosphate distribution in Swedish boreal forest soil and identify myo- and scyllo-inositol hexakisphosphates and other isomers including scyllo-inositol pentakisphosphate. Speciation of the major inositol phosphates was not altered by long-term nitrogen fertilization.

KW - inositol phosphate isomers

KW - LC-ICP-MS

KW - nitrogen fertilization

KW - phosphorus speciation

KW - phytate

KW - soil 31P NMR

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JO - Methods in Ecology and Evolution

JF - Methods in Ecology and Evolution

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ER -

LC-ICP-MS analysis of inositol phosphate isomers in soil offers improved sensitivity and fine-scale mapping of inositol phosphate distribution

Abstract

Keywords

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