Lipid fluorination enables phase separation from fluid phospholipid bilayers

Simon J. Webb, Kevin Greenaway, Marzieh Bayati, Laurent Trembleau

    Research output: Contribution to journalArticlepeer-review

    Abstract

    To probe the effect of lipid fluorination on the formation of lipid domains in phospholipid bilayers, several new fluorinated and non-fluorinated synthetic lipids were synthesised, and the extent of phase separation of these lipids from phospholipid bilayers of different compositions was determined. At membrane concentrations as low as 1% mol/mol, both fluorinated and non-fluorinated lipids were observed to phase separate from a gel-phase (solid ordered) phospholipid matrix, but bilayers in a liquid disordered state caused no phase separation; if the gel-phase samples were heated above the transition temperature, then phase separation was lost. We found incorporation of perfluoroalkyl groups into the lipid enhanced phase separation, to such an extent that phase separation was observed from cholesterol containing bilayers in the liquid ordered phase. © The Royal Society of Chemistry 2006.
    Original languageEnglish
    Pages (from-to)2399-2407
    Number of pages8
    JournalOrganic and Biomolecular Chemistry
    Volume4
    Issue number12
    DOIs
    Publication statusPublished - 2006

    Keywords

    • Membrane (bilayer, phospholipid
    • lipid fluorination enables phase sepn. from fluid phospholipid bilayers)
    • Phospholipids Role: BSU (Biological study, unclassified), BIOL (Biological study) (bilayer
    • Phase separation (lipid fluorination enables phase sepn. from fluid phospholipid bilayers)
    • Phosphatidylcholines Role: BSU (Biological study, unclassified), PRP (Properties), BIOL (Biological study) (lipid fluorination enables phase sepn. from fluid phospholipid bilayers)
    • lipid fluorination phospholipid bilayer phase sepn

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