Abstract
Significant regulation of gene expression is mediated at the translation level. Here, we describe
protocols for imaging and analysis of translation at single mRNA resolution in both fixed and living
Drosophila embryos. These protocols use the SunTag system, in which the protein of interest is
visualized by inserting a peptide array that is recognized by a single chain antibody. Simultaneous
detection of individual mRNAs using the MS2/MCP system or by smFISH allows translation sites to be
identified and quantified.
protocols for imaging and analysis of translation at single mRNA resolution in both fixed and living
Drosophila embryos. These protocols use the SunTag system, in which the protein of interest is
visualized by inserting a peptide array that is recognized by a single chain antibody. Simultaneous
detection of individual mRNAs using the MS2/MCP system or by smFISH allows translation sites to be
identified and quantified.
Original language | English |
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Journal | STAR protocols |
Publication status | Accepted/In press - 19 Aug 2021 |
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Dive into the research topics of 'Live and fixed imaging of translation sites at single mRNA resolution in the Drosophila embryo'. Together they form a unique fingerprint.Datasets
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Star_Protocols_Vinter_fixed_dataset
Vinter, D. (Creator), Mendeley Data, 6 Sept 2021
DOI: 10.17632/3h73rbvbt9.1, https://data.mendeley.com/datasets/3h73rbvbt9
Dataset
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Star_Protocols_Vinter_live_data
Vinter, D. (Creator), Mendeley Data, 6 Sept 2021
DOI: 10.17632/5vs879zvz9.1, https://data.mendeley.com/datasets/5vs879zvz9
Dataset