Live cell linear dichroism imaging reveals extensive membrane ruffling within the docking structure of natural killer cell immune synapses

Richard K P Benninger; Bruno Vanherberghen; Stephen Young; Sabrina B. Taner; Fiona J. Culley; Tim Schnyder; Mark A A Neil; Daniel Wüstner; Paul M W French; Daniel M. Davis; Björn Önfelt

doi:10.1016/j.bpj.2008.10.005

Live cell linear dichroism imaging reveals extensive membrane ruffling within the docking structure of natural killer cell immune synapses

Richard K P Benninger, Bruno Vanherberghen, Stephen Young, Sabrina B. Taner, Fiona J. Culley, Tim Schnyder, Mark A A Neil, Daniel Wüstner, Paul M W French, Daniel M. Davis, Björn Önfelt

Research output: Contribution to journal › Article › peer-review

Abstract

We have applied fluorescence imaging of two-photon linear dichroism to measure the subresolution organization of the cell membrane during formation of the activating (cytolytic) natural killer (NK) cell immune synapse (IS). This approach revealed that the NK cell plasma membrane is convoluted into ruffles at the periphery, but not in the center of a mature cytolytic NK cell IS. Time-lapse imaging showed that the membrane ruffles formed at the initial point of contact between NK cells and target cells and then spread radialy across the intercellular contact as the size of the IS increased, becoming absent from the center of the mature synapse. Understanding the role of such extensive membrane ruffling in the assembly of cytolytic synapses is an intriguing new goal. © 2009 by the biophysical Society.

Original language	English
Pages (from-to)	L13-L15
Journal	BIOPHYSICAL JOURNAL
Volume	96
Issue number	2
DOIs	https://doi.org/10.1016/j.bpj.2008.10.005
Publication status	Published - 21 Jan 2009

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http://download.cell.com/biophysj/pdf/PIIS0006349508000490.pdf?intermediate=true

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Benninger, R. K. P., Vanherberghen, B., Young, S., Taner, S. B., Culley, F. J., Schnyder, T., Neil, M. A. A., Wüstner, D., French, P. M. W., Davis, D. M., & Önfelt, B. (2009). Live cell linear dichroism imaging reveals extensive membrane ruffling within the docking structure of natural killer cell immune synapses. BIOPHYSICAL JOURNAL, 96(2), L13-L15. https://doi.org/10.1016/j.bpj.2008.10.005

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title = "Live cell linear dichroism imaging reveals extensive membrane ruffling within the docking structure of natural killer cell immune synapses",

abstract = "We have applied fluorescence imaging of two-photon linear dichroism to measure the subresolution organization of the cell membrane during formation of the activating (cytolytic) natural killer (NK) cell immune synapse (IS). This approach revealed that the NK cell plasma membrane is convoluted into ruffles at the periphery, but not in the center of a mature cytolytic NK cell IS. Time-lapse imaging showed that the membrane ruffles formed at the initial point of contact between NK cells and target cells and then spread radialy across the intercellular contact as the size of the IS increased, becoming absent from the center of the mature synapse. Understanding the role of such extensive membrane ruffling in the assembly of cytolytic synapses is an intriguing new goal. {\textcopyright} 2009 by the biophysical Society.",

author = "Benninger, {Richard K P} and Bruno Vanherberghen and Stephen Young and Taner, {Sabrina B.} and Culley, {Fiona J.} and Tim Schnyder and Neil, {Mark A A} and Daniel W{\"u}stner and French, {Paul M W} and Davis, {Daniel M.} and Bj{\"o}rn {\"O}nfelt",

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doi = "10.1016/j.bpj.2008.10.005",

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Benninger, RKP, Vanherberghen, B, Young, S, Taner, SB, Culley, FJ, Schnyder, T, Neil, MAA, Wüstner, D, French, PMW, Davis, DM & Önfelt, B 2009, 'Live cell linear dichroism imaging reveals extensive membrane ruffling within the docking structure of natural killer cell immune synapses', BIOPHYSICAL JOURNAL, vol. 96, no. 2, pp. L13-L15. https://doi.org/10.1016/j.bpj.2008.10.005

TY - JOUR

T1 - Live cell linear dichroism imaging reveals extensive membrane ruffling within the docking structure of natural killer cell immune synapses

AU - Benninger, Richard K P

AU - Vanherberghen, Bruno

AU - Young, Stephen

AU - Taner, Sabrina B.

AU - Culley, Fiona J.

AU - Schnyder, Tim

AU - Neil, Mark A A

AU - Wüstner, Daniel

AU - French, Paul M W

AU - Davis, Daniel M.

AU - Önfelt, Björn

PY - 2009/1/21

Y1 - 2009/1/21

N2 - We have applied fluorescence imaging of two-photon linear dichroism to measure the subresolution organization of the cell membrane during formation of the activating (cytolytic) natural killer (NK) cell immune synapse (IS). This approach revealed that the NK cell plasma membrane is convoluted into ruffles at the periphery, but not in the center of a mature cytolytic NK cell IS. Time-lapse imaging showed that the membrane ruffles formed at the initial point of contact between NK cells and target cells and then spread radialy across the intercellular contact as the size of the IS increased, becoming absent from the center of the mature synapse. Understanding the role of such extensive membrane ruffling in the assembly of cytolytic synapses is an intriguing new goal. © 2009 by the biophysical Society.

AB - We have applied fluorescence imaging of two-photon linear dichroism to measure the subresolution organization of the cell membrane during formation of the activating (cytolytic) natural killer (NK) cell immune synapse (IS). This approach revealed that the NK cell plasma membrane is convoluted into ruffles at the periphery, but not in the center of a mature cytolytic NK cell IS. Time-lapse imaging showed that the membrane ruffles formed at the initial point of contact between NK cells and target cells and then spread radialy across the intercellular contact as the size of the IS increased, becoming absent from the center of the mature synapse. Understanding the role of such extensive membrane ruffling in the assembly of cytolytic synapses is an intriguing new goal. © 2009 by the biophysical Society.

U2 - 10.1016/j.bpj.2008.10.005

DO - 10.1016/j.bpj.2008.10.005

M3 - Article

SN - 0006-3495

VL - 96

SP - L13-L15

JO - BIOPHYSICAL JOURNAL

JF - BIOPHYSICAL JOURNAL

IS - 2

ER -

Live cell linear dichroism imaging reveals extensive membrane ruffling within the docking structure of natural killer cell immune synapses

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