Loss of prostaglandin F2α, but not thromboxane, responsiveness in pregnant human myometrium during labour

Deborah P. Fischer; Jonathon A. Hutchinson; Diane Farrar; Peter J. O'Donovan; David F. Woodward; Kay M. Marshall

doi:10.1677/JOE-07-0494

Loss of prostaglandin F2α, but not thromboxane, responsiveness in pregnant human myometrium during labour

Deborah P. Fischer, Jonathon A. Hutchinson, Diane Farrar, Peter J. O'Donovan, David F. Woodward, Kay M. Marshall

Research output: Contribution to journal › Article › peer-review

Abstract

Prostaglandins (PG) E2, PGF2α and thromboxane (TX) mediate uterine contractility by targeting prostonoid EP, FP and TP receptors respectively. The aim of this study was to elucidate the function of these receptors in isolated human myometrium taken at term gestation prior to and following labour onset. Lower segment myometrial strips were immersed in organ baths in oxygenated Krebs' solution at 37 °C and connected to isometric force transducers. After equilibration, spontaneous activity and concentration responses to PGE2, PGF2α and U46619 (a stable TX mimetic) were measured as area under the curve and expressed as a percentage of the final contraction induced by hypotonic shock. Results were expressed as arithmetic means ± S.E.M. and analysed using two-way ANOVA with Bonferroni's post hoc test. Myometrium excised at late gestation displayed the greatest spontaneous activity compared with the tissues taken during labour (P

Original language	English
Pages (from-to)	171-179
Number of pages	8
Journal	Journal of Endocrinology
Volume	197
Issue number	1
DOIs	https://doi.org/10.1677/JOE-07-0494
Publication status	Published - Apr 2008

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abstract = "Prostaglandins (PG) E2, PGF2α and thromboxane (TX) mediate uterine contractility by targeting prostonoid EP, FP and TP receptors respectively. The aim of this study was to elucidate the function of these receptors in isolated human myometrium taken at term gestation prior to and following labour onset. Lower segment myometrial strips were immersed in organ baths in oxygenated Krebs' solution at 37 °C and connected to isometric force transducers. After equilibration, spontaneous activity and concentration responses to PGE2, PGF2α and U46619 (a stable TX mimetic) were measured as area under the curve and expressed as a percentage of the final contraction induced by hypotonic shock. Results were expressed as arithmetic means ± S.E.M. and analysed using two-way ANOVA with Bonferroni's post hoc test. Myometrium excised at late gestation displayed the greatest spontaneous activity compared with the tissues taken during labour (P",

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AU - Fischer, Deborah P.

AU - Hutchinson, Jonathon A.

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AU - O'Donovan, Peter J.

AU - Woodward, David F.

AU - Marshall, Kay M.

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Loss of prostaglandin F2α, but not thromboxane, responsiveness in pregnant human myometrium during labour

Abstract

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