Low DMSO Cryopreservation of Stem Cells Enabled by Macromolecular Cryoprotectants

Kathryn A. Murray; Ruben M. F. Tomás; Matthew I. Gibson

doi:10.1021/acsabm.0c00638

Low DMSO Cryopreservation of Stem Cells Enabled by Macromolecular Cryoprotectants

Kathryn A. Murray, Ruben M. F. Tomás, Matthew I. Gibson

Materials Chemistry

The University of Warwick

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Abstract

Mesenchymal stromal (stem) cells have potential in regenerative medicine and modulating the immune system. To deliver any cell-based therapy to the patient, it must be cryopreserved, most commonly in DMSO, which impacts cell function and causes clinical side effects. Here we report the use of a synthetically scalable polyampholyte to rescue the cryopreservation of mesenchymal stromal cells in low [DMSO] cryopreservation. Flow cytometry showed retention of key markers of multipotency comparable to 10% (v/v) DMSO, and the cells could be differentiated, showing this polymer material can be used to improve, or replace, current cryopreservation strategies.

Original language	English
Pages (from-to)	5627–5632
Number of pages	6
Journal	ACS Applied Bio Materials
Volume	3
Issue number	9
Early online date	18 Aug 2020
DOIs	https://doi.org/10.1021/acsabm.0c00638
Publication status	Published - 21 Sept 2020

Keywords

cryopreservation
stem cells
polymers
polyampholyte
biomaterials

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10.1021/acsabm.0c00638

FinalFinal published version, 5.06 MBLicence: Unspecified

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abstract = "Mesenchymal stromal (stem) cells have potential in regenerative medicine and modulating the immune system. To deliver any cell-based therapy to the patient, it must be cryopreserved, most commonly in DMSO, which impacts cell function and causes clinical side effects. Here we report the use of a synthetically scalable polyampholyte to rescue the cryopreservation of mesenchymal stromal cells in low [DMSO] cryopreservation. Flow cytometry showed retention of key markers of multipotency comparable to 10% (v/v) DMSO, and the cells could be differentiated, showing this polymer material can be used to improve, or replace, current cryopreservation strategies.",

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AU - Tomás, Ruben M. F.

AU - Gibson, Matthew I.

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AB - Mesenchymal stromal (stem) cells have potential in regenerative medicine and modulating the immune system. To deliver any cell-based therapy to the patient, it must be cryopreserved, most commonly in DMSO, which impacts cell function and causes clinical side effects. Here we report the use of a synthetically scalable polyampholyte to rescue the cryopreservation of mesenchymal stromal cells in low [DMSO] cryopreservation. Flow cytometry showed retention of key markers of multipotency comparable to 10% (v/v) DMSO, and the cells could be differentiated, showing this polymer material can be used to improve, or replace, current cryopreservation strategies.

KW - cryopreservation

KW - stem cells

KW - polymers

KW - polyampholyte

KW - biomaterials

UR - https://doi.org/10.1021/acsabm.0c00638

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DO - 10.1021/acsabm.0c00638

M3 - Article

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EP - 5632

JO - ACS Applied Bio Materials

JF - ACS Applied Bio Materials

IS - 9

ER -

Low DMSO Cryopreservation of Stem Cells Enabled by Macromolecular Cryoprotectants

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