Low production of mitochondrial reactive oxygen species after anoxia and reoxygenation in turtle hearts

Amanda Bundgaard, Anja V. Gruszczyk, Hiran A. Prag, Catherine Williams, Angela Mcintyre, Ilan M. Ruhr, Andrew M. James, Gina L. J. Galli, Michael P. Murphy, Angela Fago

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Abstract

Extremely anoxia-tolerant animals, such as freshwater turtles, survive anoxia and reoxygenation without sustaining tissue damage to their hearts. In contrast, for mammals, the ischemia-reperfusion (IR) injury that leads to tissue damage during a heart attack is initiated by a burst of superoxide (O2.-) production from the mitochondrial respiratory chain upon reperfusion of ischemic tissue. Whether turtles avoid oxidative tissue damage because of an absence of mitochondrial superoxide production upon reoxygenation, or because the turtle heart is particularly protected against this damage, is unclear. Here, we investigate whether there was an increase in mitochondrial O2.- production upon the reoxygenation of anoxic red-eared slider turtle hearts in vivo and in vitro. This was done by measuring the production of H2O2, the dismutation product of O2.-, using the mitochondria-targeted mass-spectrometric probe in vivo MitoB, while in parallel assessing changes in the metabolites driving mitochondrial O2.- production succinate, ATP and ADP levels during anoxia and H2O2 consumption and production rates of isolated heart mitochondria. We found that there was no excess production of in vivo H2O2 during 1 h of reoxygenation in turtles after 3 h anoxia at room temperature, suggesting that turtle hearts most likely do not suffer oxidative injury after anoxia because their mitochondria produce no excess O2.- upon reoxygenation. Instead, our data support the conclusion that both the low levels of succinate accumulation and the maintenance of ADP levels in the anoxic turtle heart are key factors in preventing the surge of O2.- production upon reoxygenation.
Original languageEnglish
Article number jeb.245516
JournalJournal of Experimental Biology
Early online date17 Apr 2023
DOIs
Publication statusE-pub ahead of print - 17 Apr 2023

Keywords

  • Mitochondria
  • reactive oxygen species
  • oxidative damage
  • heart
  • anoxia

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