Maintenance of a Schwann-like phenotype in differentiated adipose derived stem cells requires the synergistic action of multiple growth factors

Alice E Mortimer, Alessandro Faroni, Mahmut Kilic, Adam Reid

Research output: Contribution to journalArticlepeer-review

Abstract

Differentiating human adipose derived stem cells (ASCs) towards Schwann cells produces an unstable phenotype when stimulating factors are withdrawn. Here we set out to examine the role of glial growth factor 2 (GGF-2) in the maintenance of Schwann-like cells. Human ASCs were differentiated towards Schwann cells using previously described methods. Stimulating factors were
then withdrawn such that cells either remained in media supplemented with all stimulating factors, GGF-2 alone, or underwent complete withdrawal of all factors. Each stimulating factor was also removed from the growth medium individually to assess whether any single factor is necessary in maintaining various Schwann-like characteristics. At 72 hours several outputs were measured: gene (qRT-PCR) and protein (ELISA) expression of key factors known to dictate Schwann cell phenotype and function were quantified and cell morphology analysed. Cells treated with GGF-2 alone reverted to a stem cell morphology, and did not stimulate the production of BDNF, regardless of the
concentration of GGF-2 in the growth medium. However, GGF-2 alone increased the expression of Krox20, the main transcription factor involved in myelination, relative to those cells treated with all stimulating factors. Cells lacking fibroblast growth factor were unable to maintain a Schwann-like morphology and those lacking forskolin exhibited a downregulation in BDNF production. Therefore, it
is likely that the synergistic action of multiple growth factors is required to maintain Schwann-like phenotype in differentiated ASCs.
Original languageEnglish
Article number1479137
Number of pages7
JournalStem cells international
Volume2017
Early online date16 Jul 2017
DOIs
Publication statusPublished - 2017

Keywords

  • Schwann cell
  • Glial growth factor-2
  • Adipose derived stem cell
  • Krox-20
  • BDNF

Research Beacons, Institutes and Platforms

  • Manchester Institute for Collaborative Research on Ageing

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