Major Urinary Proteins on Nanodiamond-Based Resonators Toward Artificial Olfaction

Emmanuel Scorsone, Raafa Manai, Maria J Ricatti, Marco Redaelli, Philippe Bergonzo, Krishna C Persaud, Carla Mucignat

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    A new bio-sensing platform based on major urinary
    proteins (MUPs) from the mouse as chemical recognition
    elements has been developed. The transducers were surface
    acoustic devices coated with diamond nanoparticles as an intermediate
    layer enabling covalent attachment of the proteins.
    The resulting sensors detected 2,4-Dinitrotoluene, 4-Nitrotoluene,
    and 2-Isobutyl-3-methoxypyrazine at ppb levels. The best sensor
    showed a sensitivity of 24 000 Hz · ppm−1 to 2, 4-DNT when
    grafted with the protein MUP20. Trends in the sensitivity of
    the various VOC sensors were compared with the association
    constant values Ka of the proteins to target ligands measured by
    competitive assay in liquid phase. The system is able to detect
    analytes both in liquid as well as vapor phase and indicate that
    MUPs are robust bio-recognition elements that can be utilized in
    artificial olfaction applications.
    Original languageUndefined
    Pages (from-to)6543-6550
    Number of pages8
    JournalIEEE Sensors Journal
    Issue number17
    Early online date27 Jun 2016
    Publication statusPublished - 3 Aug 2016


    • Nanodiamond, major urinary proteins, surface acoustic wave sensors, artificial olfaction.

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